DNA content and marker expression in human glioma explants
dc.contributor.author | McKeever, Paul E. | en_US |
dc.contributor.author | Davenport, Robertson D. | en_US |
dc.date.accessioned | 2006-09-11T18:44:19Z | |
dc.date.available | 2006-09-11T18:44:19Z | |
dc.date.issued | 1987-12 | en_US |
dc.identifier.citation | Davenport, R. D.; McKeever, P. E.; (1987). "DNA content and marker expression in human glioma explants." Acta Neuropathologica 74(4): 362-365. <http://hdl.handle.net/2027.42/47230> | en_US |
dc.identifier.issn | 1432-0533 | en_US |
dc.identifier.issn | 0001-6322 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47230 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2825464&dopt=citation | en_US |
dc.description.abstract | Immunohistochemical studies of astrocytoma tissue have predominately shown fibronectin (FN) positivity restricted to vessels and glial fibrillary acidic protein (GFAP) positivity in the parenchyma. Cultured glioma cell lines, however, express both FN and GFAP. We measured the DNA content of explants of gliomas to determine if the ploidy of the FN-positive and GFAP-positive cells differed. Thirty-three explants from four high grade gliomas were cultured on slides. FN and GFAP markers were determined by double immunofluorescence. The slides were stained by the Feulgen method, the explants relocated and the DNA content measured by microdensitometry using the CAS-100 instrument. Human leukocytes applied to the slides were used as a diploid standard. Eleven GFAP-positive explants were hyperdiploid and one hypodiploid. Five FN-positive explants were diploid, three hypodiploid and ten hyperdiploid. One FN-positive explant was biclonal with aneuploid subpopulations. Two hyperdiploid explants, each of which had monoclonal histogram patterns, expressed both FN and GFAP. We conclude that most FN-positive cells, in addition to GFAP-positive cells, from cultured gliomas represent neoplastic cells. These may be present in the tumor in low numbers or may result from marker switching in culture. | en_US |
dc.format.extent | 442211 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Pathology | en_US |
dc.subject.other | Fibronectin | en_US |
dc.subject.other | Glioma | en_US |
dc.subject.other | DNA | en_US |
dc.subject.other | Cell Culture | en_US |
dc.subject.other | Glial Fibrillary Acidic Protein | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.title | DNA content and marker expression in human glioma explants | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, Medical Science I M5240/0602, University of Michigan Medical School, 1500 E. Medical Center Dr., 48109-0602, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, Medical Science I M5240/0602, University of Michigan Medical School, 1500 E. Medical Center Dr., 48109-0602, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 2825464 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47230/1/401_2004_Article_BF00687213.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00687213 | en_US |
dc.identifier.source | Acta Neuropathologica | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.