Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs
dc.contributor.author | Madison, K. C. | en_US |
dc.contributor.author | Marcelo, Cynthia L. | en_US |
dc.date.accessioned | 2006-09-11T18:44:39Z | |
dc.date.available | 2006-09-11T18:44:39Z | |
dc.date.issued | 1984-09 | en_US |
dc.identifier.citation | Marcelo, C. L.; Madison, K. C.; (1984). "Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs." Archives of Dermatological Research 276(6): 381-389. <http://hdl.handle.net/2027.42/47235> | en_US |
dc.identifier.issn | 0340-3696 | en_US |
dc.identifier.issn | 1432-069X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47235 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6595966&dopt=citation | en_US |
dc.description.abstract | A number of vitamin A analogs (retinoids) were used to manipulate the growth of epidermal keratinocytes in culture. The retinoids used were the TMMP analog of ethyl retinoate (Ro 10-9359), 13-cis retinoic acid, all trans retinoic acid and retinol (trans). These were added to primary neonatal mouse epidermal keratinocyte cultures that proliferate, stratify, and differentiate over 2–3 weeks. [ 3 H]Tdr labeling technics were used to quantitate proliferation. A histologic stain, and a four buffer protein extraction protocol, used in conjunction with polyacrylamide gel electrophoresis and fluorographic technics, were used to assess the differentiation of the cultures. Our results showed that all of the vitamin A analogs we tested inhibited keratinocyte proliferation. Quantitation of specific differentiation proteins showed that Ro 10-9359 and 13-cis retinoic acid partially inhibited the differentiation of the cultures. The Ro 10-9359 retinoid was unusual in that it increased the synthesis of keratohyalin granule-related proteins. These studies showed that inhibition of basal cell proliferation did not result in the obligatory expression of cell differentiation and that at least one of the events that is a part of epidermal keratinocyte differentiation can be separately controlled. | en_US |
dc.format.extent | 2309521 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Epidermal Keratinocyte | en_US |
dc.subject.other | Keratins | en_US |
dc.subject.other | Vitamin a Analogs | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Dermatology | en_US |
dc.subject.other | Keratohyalin Granule | en_US |
dc.subject.other | Cell Envelopes | en_US |
dc.title | Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dermatology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Dermatology, University of Michigan Medical School, Kresge I, R-6558, Box 056, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Dermatology, University of Michigan Medical School, Kresge I, R-6558, Box 056, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 6595966 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47235/1/403_2004_Article_BF00413359.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00413359 | en_US |
dc.identifier.source | Archives of Dermatological Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.