Modifications of connective tissue matrices by an enzyme extracted from cartilage
dc.contributor.author | Dziewiatkowski, Dominic D. | en_US |
dc.contributor.author | Quintarelli, G. | en_US |
dc.contributor.author | Sajdera, S. W. | en_US |
dc.date.accessioned | 2006-09-11T18:56:03Z | |
dc.date.available | 2006-09-11T18:56:03Z | |
dc.date.issued | 1968-03 | en_US |
dc.identifier.citation | Quintarelli, G.; Sajdera, S.; Dziewiatkowski, D.; (1968). "Modifications of connective tissue matrices by an enzyme extracted from cartilage." Histochemie 15(1): 1-20. <http://hdl.handle.net/2027.42/47399> | en_US |
dc.identifier.issn | 1432-119X | en_US |
dc.identifier.issn | 0948-6143 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47399 | |
dc.description.abstract | The degradative properties of an enzyme extracted from bovine costal cartilage were studied histochemically, autoradiographically, and electronmicroscopically. Previous work had indicated that this enzyme catalyzes the degradation of proteinpolysaccharide, light fraction (PP-L) from bovine costal cartilage to a proteinpolysaccharide with a lower protein content than the original substrate. Attempts were therefore made to ascertain if this protease was species specific and if proteinpolysaccharides other than those present in cartilage were susceptible to enzyme digestion. To this end, the effect of the enzyme on human fetal cartilage and jaws and the epiphyseal plates of neonatal and postnatal rats was studied. Furthermore, 35 S-H 2 SO 4 was injected into pregnant rats, the fetuses were removed and sections of them were digested with the enzyme and then autoradiographed. In the histochemical experiments Alcian Blue with MgCl 2 was used for the staining of tissue polyanions and Bromphenol Blue for the detection of free basic groups. Finally, the limbs of 20 day-old rats were utilized in electronmicroscopic studies. | en_US |
dc.format.extent | 6433187 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Anatomy | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.title | Modifications of connective tissue matrices by an enzyme extracted from cartilage | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Alabama Medical Center, Birmingham, Alabama; Regina Elena Institute for Cancer Research, Rome; The Rockefeller University, New York, N. Y.; University of Michigan, School of Dentistry, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | University of Alabama Medical Center, Birmingham, Alabama; Regina Elena Institute for Cancer Research, Rome; The Rockefeller University, New York, N. Y. | en_US |
dc.contributor.affiliationother | University of Alabama Medical Center, Birmingham, Alabama; Regina Elena Institute for Cancer Research, Rome; The Rockefeller University, New York, N. Y. | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47399/1/418_2004_Article_BF00304570.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00304570 | en_US |
dc.identifier.source | Histochemie | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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