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Modifications of connective tissue matrices by an enzyme extracted from cartilage

dc.contributor.authorDziewiatkowski, Dominic D.en_US
dc.contributor.authorQuintarelli, G.en_US
dc.contributor.authorSajdera, S. W.en_US
dc.date.accessioned2006-09-11T18:56:03Z
dc.date.available2006-09-11T18:56:03Z
dc.date.issued1968-03en_US
dc.identifier.citationQuintarelli, G.; Sajdera, S.; Dziewiatkowski, D.; (1968). "Modifications of connective tissue matrices by an enzyme extracted from cartilage." Histochemie 15(1): 1-20. <http://hdl.handle.net/2027.42/47399>en_US
dc.identifier.issn1432-119Xen_US
dc.identifier.issn0948-6143en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/47399
dc.description.abstractThe degradative properties of an enzyme extracted from bovine costal cartilage were studied histochemically, autoradiographically, and electronmicroscopically. Previous work had indicated that this enzyme catalyzes the degradation of proteinpolysaccharide, light fraction (PP-L) from bovine costal cartilage to a proteinpolysaccharide with a lower protein content than the original substrate. Attempts were therefore made to ascertain if this protease was species specific and if proteinpolysaccharides other than those present in cartilage were susceptible to enzyme digestion. To this end, the effect of the enzyme on human fetal cartilage and jaws and the epiphyseal plates of neonatal and postnatal rats was studied. Furthermore, 35 S-H 2 SO 4 was injected into pregnant rats, the fetuses were removed and sections of them were digested with the enzyme and then autoradiographed. In the histochemical experiments Alcian Blue with MgCl 2 was used for the staining of tissue polyanions and Bromphenol Blue for the detection of free basic groups. Finally, the limbs of 20 day-old rats were utilized in electronmicroscopic studies.en_US
dc.format.extent6433187 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlagen_US
dc.subject.otherMedicine & Public Healthen_US
dc.subject.otherAnatomyen_US
dc.subject.otherCell Biologyen_US
dc.subject.otherBiochemistry, Generalen_US
dc.titleModifications of connective tissue matrices by an enzyme extracted from cartilageen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumUniversity of Alabama Medical Center, Birmingham, Alabama; Regina Elena Institute for Cancer Research, Rome; The Rockefeller University, New York, N. Y.; University of Michigan, School of Dentistry, Ann Arbor, Michiganen_US
dc.contributor.affiliationotherUniversity of Alabama Medical Center, Birmingham, Alabama; Regina Elena Institute for Cancer Research, Rome; The Rockefeller University, New York, N. Y.en_US
dc.contributor.affiliationotherUniversity of Alabama Medical Center, Birmingham, Alabama; Regina Elena Institute for Cancer Research, Rome; The Rockefeller University, New York, N. Y.en_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/47399/1/418_2004_Article_BF00304570.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00304570en_US
dc.identifier.sourceHistochemieen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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