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An enrichment method for temperature-sensitive and auxotrophic mutants of yeast
dc.contributor.authorWalton, E. Fintanen_US
dc.contributor.authorCarter, Bruce L. A.en_US
dc.contributor.authorPringle, John R.en_US
dc.date.accessioned2006-09-11T19:06:22Z
dc.date.available2006-09-11T19:06:22Z
dc.date.issued1979-01en_US
dc.identifier.citationWalton, E. Fintan; Carter, Bruce L. A.; Pringle, John R.; (1979). "An enrichment method for temperature-sensitive and auxotrophic mutants of yeast." MGG Molecular & General Genetics 171(1): 111-114. <http://hdl.handle.net/2027.42/47545>en_US
dc.identifier.issn0026-8925en_US
dc.identifier.issn1617-4623en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/47545
dc.description.abstractAn enrichment procedure that exploits the difference in heat-sensitivity between exponentially growing and stationary phase cells has been developed for the isolation of yeast mutants. Enrichments of up to 12-fold for temperature-sensitive lethal mutants and of up to 15-fold for auxotrophs have been obtained with single cycles of selection. Still higher enrichments (to frequencies of greater than 90% and 80% for temperature-sensitive lethals and auxotrophs, respectively) have been obtained with multiple cycles of selection. The method requires no special parent strain, and seems adaptable to the selection of a wide variety of types of mutants.en_US
dc.format.extent320191 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlagen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherCell Biologyen_US
dc.subject.otherMicrobial Genetics and Genomicsen_US
dc.titleAn enrichment method for temperature-sensitive and auxotrophic mutants of yeasten_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Biological Sciences, The University of Michigan, 48109, Ann Arbor, Michigan, USA; Department of Genetics, University of Dublin, Trinity College, 2, Dublin, Irelanden_US
dc.contributor.affiliationumDivision of Biological Sciences, The University of Michigan, 48109, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationotherDepartment of Genetics, University of Dublin, Trinity College, 2, Dublin, Irelanden_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/47545/1/438_2004_Article_BF00274022.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00274022en_US
dc.identifier.sourceMGG Molecular & General Geneticsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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