Recombination-induced suppression of cell division following P1-mediated generalized transduction in Klebsiella aerogenes
dc.contributor.author | Sambucetti, Lidia C. | en_US |
dc.contributor.author | Bender, Robert A. | en_US |
dc.date.accessioned | 2006-09-11T19:06:46Z | |
dc.date.available | 2006-09-11T19:06:46Z | |
dc.date.issued | 1983-03 | en_US |
dc.identifier.citation | Bender, Robert A.; Sambucetti, Lidia C.; (1983). "Recombination-induced suppression of cell division following P1-mediated generalized transduction in Klebsiella aerogenes ." MGG Molecular & General Genetics 189(2): 263-268. <http://hdl.handle.net/2027.42/47551> | en_US |
dc.identifier.issn | 1617-4623 | en_US |
dc.identifier.issn | 0026-8925 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47551 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6343791&dopt=citation | en_US |
dc.description.abstract | Klebsiella aerogenes recombinants resulting from bacteriophage P1-mediated generalized transduction failed to increase in number for approximately six generations after transduction. Nevertheless these recombinants continued to grow and became sensitive to penicillin after a transient resistance, suggesting that the cells were growing as long, non-dividing filaments. When filamentous cells were isolated from transduced cultures by gradient centrifugation, recombinants were 1000-fold more frequent among the filaments than among the normal-sized cells. The suppression of cell-division lasted for six generations whether markers near the origin ( gln, ilv ) or terminus ( his, trp ) of chromosome replication were used, despite a 50-fold difference in transduction frequencies for these markers. The suppression of cell division was a host response to recombination rather than to P1 invasion since cells lysogenized by P1 in these same experiments showed only a short (two generation) suppression of cell division. We speculate that the suppression of cell-division is an SOS response triggered by the degraded DNA not incorporated in the final recombinant. We demonstrate that both the filamentation and the transient penicillin resistance of recombinant cells can be exploited to enrich greatly for recombinants, raising transduction frequencies to as high as 10 -3 . | en_US |
dc.format.extent | 633369 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag GmbH & Co. KG | en_US |
dc.subject.other | Microbial Genetics and Genomics | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.title | Recombination-induced suppression of cell division following P1-mediated generalized transduction in Klebsiella aerogenes | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Cellular and Molecular Biology, Division of Biological Sciences, University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Molecular Biology, Albert Einstein College of Medicine, 10461, Bronx, NY, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 6343791 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47551/1/438_2004_Article_BF00337815.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00337815 | en_US |
dc.identifier.source | MGG Molecular & General Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.