Molecular cloning and expression of the biodegradative threonine dehydratase gene ( tdc ) of Escherichia coli K12
dc.contributor.author | Goss, Thomas J. | en_US |
dc.contributor.author | Datta, Prasanta | en_US |
dc.date.accessioned | 2006-09-11T19:07:32Z | |
dc.date.available | 2006-09-11T19:07:32Z | |
dc.date.issued | 1985-11 | en_US |
dc.identifier.citation | Goss, Thomas J.; Datta, Prasanta; (1985). "Molecular cloning and expression of the biodegradative threonine dehydratase gene ( tdc ) of Escherichia coli K12." MGG Molecular & General Genetics 201(2): 308-314. <http://hdl.handle.net/2027.42/47562> | en_US |
dc.identifier.issn | 1617-4623 | en_US |
dc.identifier.issn | 0026-3925 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47562 | |
dc.description.abstract | The biodegradative threonine dehydratase gene ( tdc ) of Escherichia coli was cloned by isolating a dehydratase-negative mutant after Tn5 mutagenesis, cloning the tdc ::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two distinct procaryotic promoterlike elements each containing a potential CAP-binding site and AT-rich regions, and a Shine-Dalgarno sequence. One of these putative promoters, P 2 , was located immediately upstream from the tdc coding region, and a second, P 1 , was approximately 1 kilobase upstream from P 2 . Deletion of the potential CAP-binding site from P 1 prevented tdc gene expression. However, removal of P 2 and a large segment of the upstream DNA had no discernible effect on dehydratase synthesis. A 936-base pair open reading frame was found between P 1 and the tdc coding region, which produced a polypeptide of about 32 kilodaltons. The data suggest that P 1 , and not P 2 , is necessary for tdc gene expression, and that the DNA sequences coding for the 32 KD polypeptide and threonine dehydratase are part of a single transcriptional unit. | en_US |
dc.format.extent | 966903 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Microbial Genetics and Genomics | en_US |
dc.title | Molecular cloning and expression of the biodegradative threonine dehydratase gene ( tdc ) of Escherichia coli K12 | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Box 034, 48109-0010, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Box 034, 48109-0010, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47562/1/438_2004_Article_BF00425676.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00425676 | en_US |
dc.identifier.source | MGG Molecular & General Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.