A point mutation in the Nul gene of bacteriophage λ facilitates phage growth in Escherichia coli with himA and gyrB mutations

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dc.contributor.author Friedman, David I. en_US
dc.contributor.author Eades, Linda J. en_US
dc.contributor.author Alessi, Diane M. en_US
dc.contributor.author Granston, Andrew E. en_US
dc.date.accessioned 2006-09-11T19:07:53Z
dc.date.available 2006-09-11T19:07:53Z
dc.date.issued 1988-04 en_US
dc.identifier.citation Granston, Andrew E.; Alessi, Diane M.; Eades, Linda J.; Friedman, David I.; (1988). "A point mutation in the Nul gene of bacteriophage λ facilitates phage growth in Escherichia coli with himA and gyrB mutations." MGG Molecular & General Genetics 212(1): 149-156. <http://hdl.handle.net/2027.42/47567> en_US
dc.identifier.issn 0026-8925 en_US
dc.identifier.issn 1617-4623 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/47567
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2836702&dopt=citation en_US
dc.description.abstract A mutant of λ was isolated that grows in the Escherichia coli himAΔ/gyrB-him320 (Ts) double mutant at 42°C; conditions which are non-permissive for wild-type λ growth. The responsible mutation, ohm1 , alters the 40th codon of the Nul reading frame. The Nul and A gene products comprise the terminase protein which cleaves concatameric DNA into unit-length phage genomes during DNA packaging. The Nul-ohm1 gene product acts in trans to support λ growth in the double himA/gyrB mutant, and λ cos154 growth in the single himA mutant. The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA: protein interactions that occur at the initiation of packaging. en_US
dc.format.extent 848461 bytes
dc.format.extent 3115 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher Springer-Verlag en_US
dc.subject.other Life Sciences en_US
dc.subject.other DNA Packaging en_US
dc.subject.other Cell Biology en_US
dc.subject.other DNA Gyrase en_US
dc.subject.other Terminase en_US
dc.subject.other Integration Host Factor en_US
dc.subject.other Biochemistry, General en_US
dc.subject.other Microbial Genetics and Genomics en_US
dc.subject.other Cos en_US
dc.title A point mutation in the Nul gene of bacteriophage λ facilitates phage growth in Escherichia coli with himA and gyrB mutations en_US
dc.subject.hlbsecondlevel Genetics en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA en_US
dc.contributor.affiliationum Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA en_US
dc.contributor.affiliationum Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA en_US
dc.contributor.affiliationum Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA en_US
dc.contributor.affiliationumcampus Ann Arbor en_US
dc.identifier.pmid 2836702 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/47567/1/438_2004_Article_BF00322458.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1007/BF00322458 en_US
dc.identifier.source MGG Molecular & General Genetics en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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