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Isolation of DNA sequences on human chromosome 21 by application of a recombination-based assay to DNA from flow-sorted chromosomes

dc.contributor.authorTantravahi, Umadevien_US
dc.contributor.authorDrabkin, Harry A.en_US
dc.contributor.authorStewart, Gordon D.en_US
dc.contributor.authorMcNeil, Gerarden_US
dc.contributor.authorPatterson, Daviden_US
dc.contributor.authorRoy, Sayonen_US
dc.contributor.authorLalande, Marcen_US
dc.contributor.authorLatt, Samuel A.en_US
dc.contributor.authorKurnit, David M.en_US
dc.contributor.authorKeuren, Margaret L.en_US
dc.date.accessioned2006-09-11T19:12:10Z
dc.date.available2006-09-11T19:12:10Z
dc.date.issued1988-07en_US
dc.identifier.citationTantravahi, Umadevi; Stewart, Gordon D.; Keuren, Margaret; McNeil, Gerard; Roy, Sayon; Patterson, David; Drabkin, Harry; Lalande, Marc; Kurnit, David M.; Latt, Samuel A.; (1988). "Isolation of DNA sequences on human chromosome 21 by application of a recombination-based assay to DNA from flow-sorted chromosomes." Human Genetics 79(3): 196-202. <http://hdl.handle.net/2027.42/47623>en_US
dc.identifier.issn1432-1203en_US
dc.identifier.issn0340-6717en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/47623
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3402991&dopt=citationen_US
dc.description.abstractBy merging two efficient technologies, bivariate flow sorting of human metaphase chromosomes and a recombination-based assay for sequence complexity, we isolated 28 cloned DNA segments homologous to loci on human chromosome 21. Subregional mapping of these DNA segments with a somatic cell hybrid panel showed that 26 of the 28 cloned DNA sequences are distributed along the long arm of chromosome 21, while the other 2 hybridize with sequences on the short arm of both chromosome 21 and other chromosomes. This new collection of probes homologous to chromosome 21 should facilitate molecular analyses of trisomy 21 by providing DNA probes for the linkage map of chromosome 21, for studies of nondisjunction, for chromosome walking in clinically relevant subregions of chromosome 21, and for the isolation of genes on chromosome 21 following the screening of cDNA libraries.en_US
dc.format.extent737247 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlagen_US
dc.subject.otherBiomedicineen_US
dc.subject.otherMetabolic Diseasesen_US
dc.subject.otherInternal Medicineen_US
dc.subject.otherMolecular Medicineen_US
dc.subject.otherHuman Geneticsen_US
dc.titleIsolation of DNA sequences on human chromosome 21 by application of a recombination-based assay to DNA from flow-sorted chromosomesen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumHoward Hughes Medical Institute at the University of Michigan Medical Center, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumHoward Hughes Medical Institute at the University of Michigan Medical Center, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumHoward Hughes Medical Institute at the University of Michigan Medical Center, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherGenetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USAen_US
dc.contributor.affiliationotherEleanor Roosevelt Institute for Cancer Research, University of Colorado Health Sciences Center, 80206, Denver, CO, USAen_US
dc.contributor.affiliationotherGenetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USAen_US
dc.contributor.affiliationotherGenetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USAen_US
dc.contributor.affiliationotherEleanor Roosevelt Institute for Cancer Research, University of Colorado Health Sciences Center, 80206, Denver, CO, USAen_US
dc.contributor.affiliationotherGenetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA; Howard Hughes Medical Institute at The Children's Hospital, 02115, Boston, MA, USA; Department of Genetics, Harvard Medical School, 02115, Boston, MA, USAen_US
dc.contributor.affiliationotherGenetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid3402991en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/47623/1/439_2004_Article_BF00366237.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00366237en_US
dc.identifier.sourceHuman Geneticsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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