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Nocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronization

dc.contributor.authorCooper, Stephenen_US
dc.contributor.authorIyer, Geethaen_US
dc.contributor.authorTarquini, Michaelen_US
dc.contributor.authorBissett, Patricken_US
dc.date.accessioned2006-09-11T19:16:57Z
dc.date.available2006-09-11T19:16:57Z
dc.date.issued2006-05en_US
dc.identifier.citationCooper, Stephen; Iyer, Geetha; Tarquini, Michael; Bissett, Patrick; (2006). "Nocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronization." Cell and Tissue Research 324(2): 237-242. <http://hdl.handle.net/2027.42/47691>en_US
dc.identifier.issn0302-766Xen_US
dc.identifier.issn1432-0878en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/47691
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16432713&dopt=citationen_US
dc.description.abstractIt has been predicted that nocodazole-inhibited cells are not synchronized because nocodazole-arrested cells with a G2-phase amount of DNA would not have a narrow cell-size range reflecting the cell size of some specific, presumably G2-phase, cell-cycle age. Size measurements of nocodazole-inhibited cells now fully confirm this prediction. Further, release from nocodazole inhibition does not produce cells that move through the cell cycle mimicking the passage of normal unperturbed cells through the cell cycle. Nocodazole, an archetypal whole-culture synchronization method, can inhibit growth to produce cells with a G2-phase amount of DNA, but such cells are not synchronized. Cells produced by a selective (i.e., non-whole-culture) method not only have a specific DNA content, but also have a narrow size distribution. The current view of cell-cycle control that is based on methods that are not suitable for cell-cycle analysis must therefore be reconsidered when results are based on whole-culture synchronization.en_US
dc.format.extent143701 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlagen_US
dc.subject.otherCell Cycleen_US
dc.subject.otherSynchronizationen_US
dc.subject.otherNocodazoleen_US
dc.subject.otherMouse (Leukemic Cell Line)en_US
dc.subject.otherMembrane Elutionen_US
dc.subject.otherG2 Phaseen_US
dc.titleNocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronizationen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA,en_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA,en_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA,en_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA,en_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid16432713en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/47691/1/441_2005_Article_118.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00441-005-0118-8en_US
dc.identifier.sourceCell and Tissue Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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