Show simple item record

dc.contributor.authorChung, Haegeunen_US
dc.contributor.authorZak, Donald R.en_US
dc.contributor.authorLilleskov, Erik A.en_US
dc.date.accessioned2006-09-11T19:18:19Z
dc.date.available2006-09-11T19:18:19Z
dc.date.issued2006-02en_US
dc.identifier.citationChung, Haegeun; Zak, Donald R.; Lilleskov, Erik A.; (2006). "Fungal community composition and metabolism under elevated CO 2 and O 3 ." Oecologia 147(1): 143-154. <http://hdl.handle.net/2027.42/47711>en_US
dc.identifier.issn1432-1939en_US
dc.identifier.issn0029-8549en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/47711
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16205953&dopt=citationen_US
dc.description.abstractAtmospheric CO 2 and O 3 concentrations are increasing due to human activity and both trace gases have the potential to alter C cycling in forest ecosystems. Because soil microorganisms depend on plant litter as a source of energy for metabolism, changes in the amount or the biochemistry of plant litter produced under elevated CO 2 and O 3 could alter microbial community function and composition. Previously, we have observed that elevated CO 2 increased the microbial metabolism of cellulose and chitin, whereas elevated O 3 dampened this response. We hypothesized that this change in metabolism under CO 2 and O 3 enrichment would be accompanied by a concomitant change in fungal community composition. We tested our hypothesis at the free-air CO 2 and O 3 enrichment (FACE) experiment at Rhinelander, Wisconsin, in which Populus tremuloides , Betula papyrifera , and Acer saccharum were grown under factorial CO 2 and O 3 treatments. We employed extracellular enzyme analysis to assay microbial metabolism, phospholipid fatty acid (PLFA) analysis to determine changes in microbial community composition, and polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE) to analyze the fungal community composition. The activities of 1,4-β-glucosidase (+37%) and 1,4,-β- N -acetylglucosaminidase (+84%) were significantly increased under elevated CO 2 , whereas 1,4-β-glucosidase activity (−25%) was significantly suppressed by elevated O 3 . There was no significant main effect of elevated CO 2 or O 3 on fungal relative abundance, as measured by PLFA. We identified 39 fungal taxonomic units from soil using DGGE, and found that O 3 enrichment significantly altered fungal community composition. We conclude that fungal metabolism is altered under elevated CO 2 and O 3 , and that there was a concomitant change in fungal community composition under elevated O 3 . Thus, changes in plant inputs to soil under elevated CO 2 and O 3 can propagate through the microbial food web to alter the cycling of C in soil.en_US
dc.format.extent777293 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlagen_US
dc.subject.otherExtracellular Enzymesen_US
dc.subject.otherSoil Microbial Communityen_US
dc.subject.otherFungal Metabolismen_US
dc.subject.otherPolymerase Chain Reaction–Denaturing Gradient Gel Electrophoresisen_US
dc.subject.otherFree-air CO 2 and O 3 Enrichmenten_US
dc.titleFungal community composition and metabolism under elevated CO 2 and O 3en_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumSchool of Natural Resources and Environment, University of Michigan, Ann Arbor, Michigan, 48109-1115, USA, ; Department of Plant Sciences, University of California, Davis, CA, 95616, USA,en_US
dc.contributor.affiliationumSchool of Natural Resources and Environment, University of Michigan, Ann Arbor, Michigan, 48109-1115, USA, ; Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, 48109-1048, USA,en_US
dc.contributor.affiliationotherUSDA Forest Service North Central Experiment Station, Houghton, Michigan, 49931, USA,en_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid16205953en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/47711/1/442_2005_Article_249.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00442-005-0249-3en_US
dc.identifier.sourceOecologiaen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record