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dc.contributor.authorSeaton, Jay J.en_US
dc.contributor.authorWang, Henry Y.en_US
dc.date.accessioned2006-09-11T19:35:06Z
dc.date.available2006-09-11T19:35:06Z
dc.date.issued1987-10en_US
dc.identifier.citationWang, Henry Y.; Seaton, Jay J.; (1987). "Increased sensitivity and discrimination in screening through an immobilized-resin microbiological assay method." Journal of Industrial Microbiology 2(4): 243-249. <http://hdl.handle.net/2027.42/47948>en_US
dc.identifier.issn0169-4146en_US
dc.identifier.issn1476-5535en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/47948
dc.description.abstractProblems with present bioactive microbial product screening techniques include low sensitivity and insufficient discrimination capabilities. These problems are addressed by our new immobilized-resin microbiological assay. This technique concentrates bioactive samples on macroporous polymeric resins that are immobilized in hydrogel beads. These beads are then subjected to elution in the wells of an agar diffusion microbiological assay medium. With a strong base anion exchanger, the sensitivity to ampicillin of the β-lactam-supersensitive Escherichia coli mutant ESS-22-31 was increased 10-fold. Similar increases in sensitivity were obtained in the detection of streptomycin using a weak acid cation exchanger with Bacillus subtilis and for cycloheximide by a neutral resin and Saccharomyces cerevisiae NRRL-Y-139. A judicious choice of resin type and eluent permitted a selective sensitivity increase based on the charge or hydrophobic nature of the desired product. This selectivity imparts a discrimination capability to the technique.en_US
dc.format.extent553898 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Society for Industrial Microbiologyen_US
dc.subject.otherMicrobial Genetics and Genomicsen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherGenetic Engineeringen_US
dc.subject.otherBioinformaticsen_US
dc.subject.otherPolymeric Resinen_US
dc.subject.otherBioscreeningen_US
dc.subject.otherBioassayen_US
dc.subject.otherAntibioticen_US
dc.subject.otherMicrobiologyen_US
dc.subject.otherChemistryen_US
dc.subject.otherBiotechnologyen_US
dc.titleIncreased sensitivity and discrimination in screening through an immobilized-resin microbiological assay methoden_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemical Engineering, The University of Michigan, 48109-2136, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, The University of Michigan, 48109-2136, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/47948/1/10295_2005_Article_BF01569546.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF01569546en_US
dc.identifier.sourceJournal of Industrial Microbiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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