Comparing recovering efficiency of immunomagnetic separation and centrifugation of mycobacteria in metalworking fluids

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dc.contributor.author Gruden, Cyndee L. en_US
dc.contributor.author Bahrman, Sarah E. en_US
dc.contributor.author Chang, Shu-Chi en_US
dc.contributor.author Anderson, Tracey I. en_US
dc.contributor.author Adriaens, Peter en_US
dc.contributor.author Khijniak, Anna I. en_US
dc.date.accessioned 2006-09-11T19:35:10Z
dc.date.available 2006-09-11T19:35:10Z
dc.date.issued 2005-12 en_US
dc.identifier.citation Chang, Shu-Chi; Anderson, Tracey I.; Bahrman, Sarah E.; Gruden, Cyndee L.; Khijniak, Anna I.; Adriaens, Peter; (2005). "Comparing recovering efficiency of immunomagnetic separation and centrifugation of mycobacteria in metalworking fluids." Journal of Industrial Microbiology & Biotechnology 32 (11-12): 629-638. <http://hdl.handle.net/2027.42/47949> en_US
dc.identifier.issn 1476-5535 en_US
dc.identifier.issn 1367-5435 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/47949
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15933873&dopt=citation en_US
dc.description.abstract The accurate detection and enumeration of Mycobacterium immunogenum in metalworking fluids (MWFs) is imperative from an occupational health and industrial fluids management perspective. We report here a comparison of immunomagnetic separation (IMS) coupled to flow-cytometric enumeration, with traditional centrifugation techniques for mycobacteria in a semisynthetic MWF. This immunolabeling involves the coating of laboratory-synthesized nanometer-scale magnetic particles with protein A, to conjugate a primary antibody (Ab), specific to Mycobacterium spp. By using magnetic separation and flow-cytometric quantification, this approach enabled much higher recovery efficiency and fluorescent light intensities in comparison to the widely applied centrifugation technique. This IMS technique increased the cell recovery efficiency by one order of magnitude, and improved the fluorescence intensity of the secondary Ab conjugate by 2-fold, as compared with traditional techniques. By employing nanometer-scale magnetic particles, IMS was found to be compatible with flow cytometry (FCM), thereby increasing cell detection and enumeration speed by up to two orders of magnitude over microscopic techniques. Moreover, the use of primary Ab conjugated magnetic nanoparticles showed better correlation between epifluorescent microscopy counts and FCM analysis than that achieved using traditional centrifugation techniques. The results strongly support the applicability of the flow-cytometric IMS for microbial detection in complex matrices. en_US
dc.format.extent 560526 bytes
dc.format.extent 3115 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher Springer-Verlag; Society for Industrial Microbiology en_US
dc.subject.other Metalworking Fluid en_US
dc.subject.other Mycobacteria en_US
dc.subject.other Immunomagnetic Separation en_US
dc.subject.other Flow Cytometry en_US
dc.subject.other Recovery en_US
dc.title Comparing recovering efficiency of immunomagnetic separation and centrifugation of mycobacteria in metalworking fluids en_US
dc.type Environmental Biotechnology en_US
dc.subject.hlbsecondlevel Biomedical Engineering en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Civil and Environmental Engineering, , University of Michigan, , , Room 116, EWRE Bldg., 1351 Beal Avenue, , Ann Arbor, , MI, , 48109, , USA, en_US
dc.contributor.affiliationum Department of Civil and Environmental Engineering, , University of Michigan, , , Room 116, EWRE Bldg., 1351 Beal Avenue, , Ann Arbor, , MI, , 48109, , USA, en_US
dc.contributor.affiliationum Department of Civil and Environmental Engineering, , University of Michigan, , , Room 116, EWRE Bldg., 1351 Beal Avenue, , Ann Arbor, , MI, , 48109, , USA, en_US
dc.contributor.affiliationum Department of Civil and Environmental Engineering, , University of Michigan, , , Room 116, EWRE Bldg., 1351 Beal Avenue, , Ann Arbor, , MI, , 48109, , USA, en_US
dc.contributor.affiliationum Department of Civil and Environmental Engineering, , University of Michigan, , , Room 116, EWRE Bldg., 1351 Beal Avenue, , Ann Arbor, , MI, , 48109, , USA, en_US
dc.contributor.affiliationother Department of Civil Engineering, , University of Toledo, , , Toledo, , OH, , USA, en_US
dc.contributor.affiliationumcampus Ann Arbor en_US
dc.identifier.pmid 15933873 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/47949/1/10295_2005_Article_238.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1007/s10295-005-0238-x en_US
dc.identifier.source Journal of Industrial Microbiology & Biotechnology en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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