Immunohistochemical Characterization of Rapid Dentin Formation Induced by Enamel Matrix Derivative

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dc.contributor.author Nakamura, Y. en_US
dc.contributor.author Ritchie, H. H. en_US
dc.contributor.author Matsumoto, K. en_US
dc.contributor.author Lyngstadaas, S. P. en_US
dc.contributor.author Slaby, I. en_US
dc.date.accessioned 2006-09-11T19:39:25Z
dc.date.available 2006-09-11T19:39:25Z
dc.date.issued 2004-09 en_US
dc.identifier.citation Nakamura, Y.; Slaby, I.; Matsumoto, K.; Ritchie, H. H.; Lyngstadaas, S. P.; (2004). "Immunohistochemical Characterization of Rapid Dentin Formation Induced by Enamel Matrix Derivative." Calcified Tissue International 75(3): 243-252. <http://hdl.handle.net/2027.42/48011> en_US
dc.identifier.issn 0171-967X en_US
dc.identifier.issn 1432-0827 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/48011
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12566040&dopt=citation en_US
dc.description.abstract The purpose of this study was to examine the pulpal expression of dentin-related proteins during enamel matrix derivative (EMD)–induced reparative dentin formation in a pulpotomy model in pig incisors. Pulpotomies were performed on 72 lower incisors in 24 adult miniature swine. The exposed pulp tissue was treated with EMD or covered with a calcium hydroxide paste (Dycal ® ). At predefined time-points, ranging from 4 days to 12 weeks, experimental teeth were extracted and examined by use of light microscopy, and expression of dentin-related proteins in the pulps was investigated by immunohistochemistry, using antibodies against type I collagen, dentin sialoprotein (DSP), sheathlin, and EMD. In all EMD-treated teeth a substantial amount of reparative dentin formation was observed. The amount of reparative dentin in calcium hydroxide–treated teeth was significantly smaller than in EMD-treated teeth ( P < 0.005) and was less effective in bridging the pulpal wounds. Immunohistochemistry demonstrated that enamel matrix proteins were present in detectable amounts at the application site for about 4 weeks. Moreover, the expression of proteins related to dentin formation in the wounded pulp tissue was about 2 weeks advanced in EMD-treated teeth. These findings demonstrate that enamel matrix molecules have the capacity to induce rapid pulpal wound healing in pulpotomized teeth, and suggest that the longevity and continued presence of enamel matrix macromolecules at the application site can be utilized to stimulate growth and repair of dentin over a period consistent with a favorable clinical outcome. en_US
dc.format.extent 430165 bytes
dc.format.extent 3115 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher Springer-Verlag en_US
dc.subject.other Immunohistochemistry en_US
dc.subject.other Enamel Matrix Derivative en_US
dc.subject.other Reparative Dentin Formation en_US
dc.subject.other Pulpal Wound Healing en_US
dc.subject.other Philosophy en_US
dc.title Immunohistochemical Characterization of Rapid Dentin Formation Induced by Enamel Matrix Derivative en_US
dc.type Article en_US
dc.subject.hlbsecondlevel Dentistry en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, USA en_US
dc.contributor.affiliationother Department of Endodontics, School of Dentistry, Showa University, 2-1-1, Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan en_US
dc.contributor.affiliationother Oral Research Laboratory, Faculty of Dentistry, University of Oslo, PO box 1109, Blindern, NO-0317 Oslo, Norway en_US
dc.contributor.affiliationother Department of Endodontics, School of Dentistry, Showa University, 2-1-1, Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan en_US
dc.contributor.affiliationother Biora AB, Medeon Science Park, Malmö SE 205-12, Sweden en_US
dc.contributor.affiliationumcampus Ann Arbor en_US
dc.identifier.pmid 12566040 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/48011/1/223_2003_Article_153.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1007/s00223-003-0153-y en_US
dc.identifier.source Calcified Tissue International en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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