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dc.contributor.authorBentley, P.en_US
dc.contributor.authorClarkson, B. H.en_US
dc.contributor.authorLiu, J.en_US
dc.contributor.authorChen, H.en_US
dc.contributor.authorWood, D.en_US
dc.contributor.authorChang, S.en_US
dc.date.accessioned2006-09-11T19:39:34Z
dc.date.available2006-09-11T19:39:34Z
dc.date.issued2006-01en_US
dc.identifier.citationChang, S.; Chen, H.; Liu, J.; Wood, D.; Bentley, P.; Clarkson, B.; (2006). "Synthesis of a Potentially Bioactive, Hydroxyapatite-Nucleating Molecule." Calcified Tissue International 78(1): 55-61. <http://hdl.handle.net/2027.42/48013>en_US
dc.identifier.issn1432-0827en_US
dc.identifier.issn0171-967Xen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/48013
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16397739&dopt=citationen_US
dc.description.abstractA human phosphophoryn (PP) cDNA was previously cloned from immature root apex total RNA in our laboratory. This cDNA comprises 2,364 bp, encoding 788 amino acids. More than 80% of the sequences are arranged as (DSS) n ( n = 1–16), DS, and NSS motifs. We hypothesize that the capability of PP to bind Ca 2+ and nucleate hydroxyapatite may depend on these repeated sequences. Two polypeptides were synthesized based on the human PP cDNA sequence to test the hypothesis. One polypeptide has the amino acid sequence DDPNSSDESNGNDD (synthetic polypeptide 1, SP1), which is from the N-terminal end of PP; the other polypeptide, DSKSDSSKSESDSS (synthetic polypeptide 2, SP2), is the PP repeated sequence motif. Phosphorylation of the polypeptides was accomplished by reacting them with adenosine triphosphate and casein kinases I and II. The ability of these molecules to cause mineralization was tested in a steady-state agarose gel system. The results show that phosphorylated SP2 (P-SP2) precipitated approximately 60% of the total Ca + PO 4 precipitated by PP. P-SP1 precipitated about 23% of that precipitated by PP and was similar to the amount precipitated in the control gel, that is, without added peptides. Transmission electron microscopy and X-ray diffraction analysis showed that the precipitate formed in the P-SP2-containing gel was hydroxyapatite. The capability of P-SP2 to nucleate Ca + PO 4 and precipitate hydroxyapatite is a result of the repeated sequence motif, which contains a high percentage of phosphorylated serine. This molecule could be used in the repair and regeneration of dental tissue.en_US
dc.format.extent404461 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Springer Science+Business Media, Inc.en_US
dc.subject.otherHumanen_US
dc.subject.otherHydroxyapatiteen_US
dc.subject.otherPhosphophorynen_US
dc.subject.otherNucleationen_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherCell Biologyen_US
dc.subject.otherEndocrinologyen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherPolypeptideen_US
dc.subject.otherOrthopedicsen_US
dc.titleSynthesis of a Potentially Bioactive, Hydroxyapatite-Nucleating Moleculeen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Cariology, Restorative Sciences, and Endodontics, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Cariology, Restorative Sciences, and Endodontics, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Cariology, Restorative Sciences, and Endodontics, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Cariology, Restorative Sciences, and Endodontics, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherDivision of Restorative Dentistry, University of Leeds, Leeds, United Kingdomen_US
dc.contributor.affiliationotherDivision of Restorative Dentistry, University of Leeds, Leeds, United Kingdomen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid16397739en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/48013/1/223_2005_Article_118.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00223-005-0118-4en_US
dc.identifier.sourceCalcified Tissue Internationalen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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