Regulation of Cementoblast Gene Expression by Inorganic Phosphate In Vitro
dc.contributor.author | Matsa-Dunn, D. | en_US |
dc.contributor.author | Somerman, Martha J. | en_US |
dc.contributor.author | Berry, J. E. | en_US |
dc.contributor.author | Swanson, E. C. | en_US |
dc.contributor.author | Foster, B. L. | en_US |
dc.contributor.author | Nociti, F. H. | en_US |
dc.contributor.author | Zhang, P. | en_US |
dc.contributor.author | Cupp, C. J. | en_US |
dc.date.accessioned | 2006-09-11T19:39:42Z | |
dc.date.available | 2006-09-11T19:39:42Z | |
dc.date.issued | 2006-02 | en_US |
dc.identifier.citation | Foster, B. L.; Nociti, F. H.; Swanson, E. C.; Matsa-Dunn, D.; Berry, J. E.; Cupp, C. J.; Zhang, P.; Somerman, M. J.; (2006). "Regulation of Cementoblast Gene Expression by Inorganic Phosphate In Vitro ." Calcified Tissue International 78(2): 103-112. <http://hdl.handle.net/2027.42/48015> | en_US |
dc.identifier.issn | 1432-0827 | en_US |
dc.identifier.issn | 0171-967X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/48015 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16467974&dopt=citation | en_US |
dc.description.abstract | Examination of mutant and knockout phenotypes with altered phosphate/pyrophosphate distribution has demonstrated that cementum, the mineralized tissue that sheathes the tooth root, is very sensitive to local levels of phosphate and pyrophosphate. The aim of this study was to examine the potential regulation of cementoblast cell behavior by inorganic phosphate (P i ). Immortalized murine cementoblasts were treated with P i in vitro , and effects on gene expression (by quantitative real-time reverse-transcriptase polymerase chain reaction [RT-PCR]) and cell proliferation (by hemacytometer count) were observed. Dose-response (0.1–10 mM) and time-course (1–48 hours) assays were performed, as well as studies including the Na-P i uptake inhibitor phosphonoformic acid. Real-time RT-PCR indicated regulation by phosphate of several genes associated with differentiation/mineralization. A dose of 5 mM P i upregulated genes including the SIBLING family genes osteopontin ( Opn , >300% of control) and dentin matrix protein-1 ( Dmp-1 , >3,000% of control). Another SIBLING family member, bone sialoprotein ( Bsp ), was downregulated, as were osteocalcin ( Ocn ) and type I collagen ( Col1 ). Time-course experiments indicated that these genes responded within 6–24 hours. Time-course experiments also indicated rapid regulation (by 6 hours) of genes concerned with phosphate/pyrophosphate homeostasis, including the mouse progressive ankylosis gene ( Ank ), plasma cell membrane glycoprotein-1 ( Pc-1 ), tissue nonspecific alkaline phosphatase ( Tnap ), and the Pit1 Na-P i cotransporter. Phosphate effects on cementoblasts were further shown to be uptake-dependent and proliferation-independent. These data suggest regulation by phosphate of multiple genes in cementoblasts in vitro . During formation, phosphate and pyrophosphate may be important regulators of cementoblast functions including maturation and regulation of matrix mineralization. | en_US |
dc.format.extent | 754150 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer Science+Business Media, Inc. | en_US |
dc.subject.other | SIBLING Gene | en_US |
dc.subject.other | Phosphate | en_US |
dc.subject.other | Cementoblast | en_US |
dc.subject.other | Endocrinology | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Pyrophosphate | en_US |
dc.subject.other | Osteopontin | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Orthopedics | en_US |
dc.subject.other | Life Sciences | en_US |
dc.title | Regulation of Cementoblast Gene Expression by Inorganic Phosphate In Vitro | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Periodontics, School of Dentistry, University of Washington, Seattle, WA, USA | en_US |
dc.contributor.affiliationother | Department of Periodontics, School of Dentistry, University of Washington, Seattle, WA, USA | en_US |
dc.contributor.affiliationother | Nestlé Purina Research, St. Louis, MO, USA | en_US |
dc.contributor.affiliationother | Nestlé Purina Research, St. Louis, MO, USA | en_US |
dc.contributor.affiliationother | Department of Periodontics, School of Dentistry, University of Washington, Seattle, WA, USA | en_US |
dc.contributor.affiliationother | Department of Periodontics, School of Dentistry, University of Washington, Seattle, WA, USA; Division of Periodontics, Department of Prosthodontics/Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, Brazil | en_US |
dc.contributor.affiliationother | Department of Periodontics, School of Dentistry, University of Washington, Seattle, WA, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 16467974 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/48015/1/223_2005_Article_184.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s00223-005-0184-7 | en_US |
dc.identifier.source | Calcified Tissue International | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.