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dc.contributor.authorCandia, Oscar A.en_US
dc.contributor.authorReinach, Peter S.en_US
dc.contributor.authorSiegel, George J.en_US
dc.date.accessioned2006-09-11T19:40:23Z
dc.date.available2006-09-11T19:40:23Z
dc.date.issued1975-12en_US
dc.identifier.citationReinach, Peter S.; Candia, Oscar A.; Siegel, George J.; (1975). "Lithium transport across isolated frog skin epithelium." The Journal of Membrane Biology 25(1): 75-92. <http://hdl.handle.net/2027.42/48025>en_US
dc.identifier.issn1432-1424en_US
dc.identifier.issn0022-2631en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/48025
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1082512&dopt=citationen_US
dc.description.abstractTransepithelial Li + influx was studied in the isolated epithelium from abdominal skin of Rana catesbeiana . With Na + -Ringer's as inside medium and Li + -Ringer's as outside medium, the Li + influx across the epithelium was 15.6 μA/cm 2 . This influx was considerably reduced by removal of either Na + or K + from the inside bath or by the addition of ouabain or amiloride. Epithelial K + or Na + concentration was respectively lower in epithelia bathed in K + -free Ringer's or Na + -free Ringer's. In conditions of negligible Na + transport, a 20 mm Li + gradient (out→in) produced across the short-circuited epithelium a Li + influx of 11.8 μA/cm 2 and a mean short-circuit current of 10.2 μA/cm 2 . The same Li + gradient in the opposite direction produced a Li + outflux of only 1.9 μA/cm 2 . With equal Li + concentration (10.3 and 20.6 mm ) on both sides of the epithelium, plus Na + in the inside solution only, a stable Li + -dependent short-circuit current was observed. Net Li + movement (out→in) was also indirectly determined in the presence of an opposing Li + gradient. Although Li + does not substitute for Na + as an activator of the (Na + +K + )-ATPase from frog skin epithelium, Li + influx appears to be related to Na + −K + pump activity. It is proposed that the permeability of the “outer barrier” to Na + and Li + is regulated by the electrical gradient produced by electrogenic Na + −K + pumps located in the membrane of the deeper epithelial cells.en_US
dc.format.extent1025912 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Springer-Verlag New York Inc.en_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherHuman Physiologyen_US
dc.subject.otherBiochemistry, Generalen_US
dc.titleLithium transport across isolated frog skin epitheliumen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Ophthalmology, Mount Sinai School of Medicine of the City University of New York, Fifth Avenue and 100th Street, 10029, New York, New York; Department of Neurology, University of Michigan Medical Center, 48104, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Ophthalmology, Mount Sinai School of Medicine of the City University of New York, Fifth Avenue and 100th Street, 10029, New York, New York; Department of Neurology, University of Michigan Medical Center, 48104, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Ophthalmology, Mount Sinai School of Medicine of the City University of New York, Fifth Avenue and 100th Street, 10029, New York, New York; Department of Neurology, University of Michigan Medical Center, 48104, Ann Arbor, Michiganen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid1082512en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/48025/1/232_2005_Article_BF01868569.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF01868569en_US
dc.identifier.sourceThe Journal of Membrane Biologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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