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Effects of ethacrynic acid on ion transport and energy metabolism in slices of avian salt gland and of mammalian liver and kidney cortex

dc.contributor.authorErnst, Stephen A.en_US
dc.contributor.authorRossum, George D. V.en_US
dc.date.accessioned2006-09-11T19:40:31Z
dc.date.available2006-09-11T19:40:31Z
dc.date.issued1978-11en_US
dc.identifier.citationRossum, George D. V.; Ernst, Stephen A.; (1978). "Effects of ethacrynic acid on ion transport and energy metabolism in slices of avian salt gland and of mammalian liver and kidney cortex." The Journal of Membrane Biology 43 (2-3): 251-275. <http://hdl.handle.net/2027.42/48027>en_US
dc.identifier.issn1432-1424en_US
dc.identifier.issn0022-2631en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/48027
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=712819&dopt=citationen_US
dc.description.abstractEthacrynic acid greatly inhibited net transport of ions and aerobic, energyconserving metabolism in slices of avian salt gland, rat liver, and rat and guinea-pig kidney cortex. The effects of increasing concentrations of ethacrynic acid on the transport of Na + , K + and Cl − ran closely parallel to its effects on tissue ATP levels and respiration. The concentration needed for maximal inhibition of transport reduced ATP levels by 80–90%. Respiration was reduced by 80–90% in salt gland and kidney cortex, and by a maximum of 30% in liver slices. The effects of low concentrations of ethacrynic acid required time to become fully manifest in some tissues, and the development of transport inhibition followed a similar course to decline of respiration and ATP levels. Ca 2+ extrusion by liver cells was inhibited by ethacrynic acid. The concentration dependence of the inhibition was similar to that shown by the other transport systems inhibited. There was no distinction evident between the sensitivity of Na + extrusion and of K + accumulation to the diuretic. Lactate production increased as respiration decreased in the presence of increasing concentrations of ethacrynic acid. We conclude that ethacrynic acid acted primarily as an inhibitor of mitochondrial respiration and ATP synthesis in the tissue slices, and that inhibition of ion transport was a nonspecific consequence of the failure of the energy supply.en_US
dc.format.extent1308538 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Springer-Verlag New York Inc.en_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherHuman Physiologyen_US
dc.subject.otherLife Sciencesen_US
dc.titleEffects of ethacrynic acid on ion transport and energy metabolism in slices of avian salt gland and of mammalian liver and kidney cortexen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pharmacology and Anatomy, Temple University School of Medicine, 19140, Philadelphia, PA; Department of Anatomy, The University of Michigan Medical School, Medical Sciences II Building, 48109, Ann Arbor, MIen_US
dc.contributor.affiliationotherDepartment of Pharmacology and Anatomy, Temple University School of Medicine, 19140, Philadelphia, PAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid712819en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/48027/1/232_2005_Article_BF01933482.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF01933482en_US
dc.identifier.sourceThe Journal of Membrane Biologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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