Effects of ethacrynic acid on ion transport and energy metabolism in slices of avian salt gland and of mammalian liver and kidney cortex
dc.contributor.author | Ernst, Stephen A. | en_US |
dc.contributor.author | Rossum, George D. V. | en_US |
dc.date.accessioned | 2006-09-11T19:40:31Z | |
dc.date.available | 2006-09-11T19:40:31Z | |
dc.date.issued | 1978-11 | en_US |
dc.identifier.citation | Rossum, George D. V.; Ernst, Stephen A.; (1978). "Effects of ethacrynic acid on ion transport and energy metabolism in slices of avian salt gland and of mammalian liver and kidney cortex." The Journal of Membrane Biology 43 (2-3): 251-275. <http://hdl.handle.net/2027.42/48027> | en_US |
dc.identifier.issn | 1432-1424 | en_US |
dc.identifier.issn | 0022-2631 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/48027 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=712819&dopt=citation | en_US |
dc.description.abstract | Ethacrynic acid greatly inhibited net transport of ions and aerobic, energyconserving metabolism in slices of avian salt gland, rat liver, and rat and guinea-pig kidney cortex. The effects of increasing concentrations of ethacrynic acid on the transport of Na + , K + and Cl − ran closely parallel to its effects on tissue ATP levels and respiration. The concentration needed for maximal inhibition of transport reduced ATP levels by 80–90%. Respiration was reduced by 80–90% in salt gland and kidney cortex, and by a maximum of 30% in liver slices. The effects of low concentrations of ethacrynic acid required time to become fully manifest in some tissues, and the development of transport inhibition followed a similar course to decline of respiration and ATP levels. Ca 2+ extrusion by liver cells was inhibited by ethacrynic acid. The concentration dependence of the inhibition was similar to that shown by the other transport systems inhibited. There was no distinction evident between the sensitivity of Na + extrusion and of K + accumulation to the diuretic. Lactate production increased as respiration decreased in the presence of increasing concentrations of ethacrynic acid. We conclude that ethacrynic acid acted primarily as an inhibitor of mitochondrial respiration and ATP synthesis in the tissue slices, and that inhibition of ion transport was a nonspecific consequence of the failure of the energy supply. | en_US |
dc.format.extent | 1308538 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Human Physiology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.title | Effects of ethacrynic acid on ion transport and energy metabolism in slices of avian salt gland and of mammalian liver and kidney cortex | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology and Anatomy, Temple University School of Medicine, 19140, Philadelphia, PA; Department of Anatomy, The University of Michigan Medical School, Medical Sciences II Building, 48109, Ann Arbor, MI | en_US |
dc.contributor.affiliationother | Department of Pharmacology and Anatomy, Temple University School of Medicine, 19140, Philadelphia, PA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 712819 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/48027/1/232_2005_Article_BF01933482.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01933482 | en_US |
dc.identifier.source | The Journal of Membrane Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.