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dc.contributor.authorDyer, Kimberly D.en_US
dc.contributor.authorZhang, Jianzhien_US
dc.contributor.authorRosenberg, Helene F.en_US
dc.date.accessioned2006-09-11T19:42:42Z
dc.date.available2006-09-11T19:42:42Z
dc.date.issued2004-11en_US
dc.identifier.citationDyer, Kimberly D.; Rosenberg, Helene F.; Zhang, Jianzhi; (2004). "Isolation, Characterization, and Evolutionary Divergence of Mouse RNase 6: Evidence for Unusual Evolution in Rodents." Journal of Molecular Evolution 59(5): 657-665. <http://hdl.handle.net/2027.42/48058>en_US
dc.identifier.issn0022-2844en_US
dc.identifier.issn1432-1432en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/48058
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15693621&dopt=citationen_US
dc.description.abstractThe evolution of the ribonuclease A (RNase A) vertebrate-specific enzyme family is interesting in that specific gene lineages appear to be responding to unique selective pressures in wildly diverse manners to generate proteins that are capable of reducing the infectivity of viruses, killing systemic pathogens, and inducing the growth of blood vessels all while maintaining the signature motifs of a ribonuclease. In this paper, we present the DNA sequence and gene structure of Mus musculus RNase 6 and examine the expression pattern and enzymatic activity of the recombinant protein. M. musculus RNase 6 has a limited expression pattern compared to human RNase 6 and is an efficient ribonuclease, with a catalytic efficiency 17-fold higher than that of human protein. Evo- lutionary analysis reveals that RNase 6 was subject to unusual evolutionary forces ( d N / d S  = 1.2) in an ancestral rodent lineage before the separation of Mus and Rattus . However, more recent evolution of rodent RNase 6 has been relatively conserved, with an average d N / d S of 0.66. These data suggest that the ancestral rodent RNase 6 was subject to accelerated evolution, resulting in the conserved modern gene, which most likely plays an important role in mouse physiology.en_US
dc.format.extent295070 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Springer Science + Business Media Inc.en_US
dc.subject.otherRNase K6en_US
dc.subject.otherRNase 6en_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherMicrobiologyen_US
dc.subject.otherRNase a Superfamilyen_US
dc.subject.otherHost Defenseen_US
dc.subject.otherRibonucleaseen_US
dc.subject.otherPlant Sciencesen_US
dc.subject.otherCell Biologyen_US
dc.titleIsolation, Characterization, and Evolutionary Divergence of Mouse RNase 6: Evidence for Unusual Evolution in Rodentsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, 48109, USAen_US
dc.contributor.affiliationotherEosinophil Biology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, Bethesda, MD, 20892, USAen_US
dc.contributor.affiliationotherEosinophil Biology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, Bethesda, MD, 20892, USAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid15693621en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/48058/1/239_2004_Article_2657.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00239-004-2657-0en_US
dc.identifier.sourceJournal of Molecular Evolutionen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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