4-(4-nitrobenzyl)pyridine tests for alkylating agents following chemical oxidative activation
dc.contributor.author | Thomas, John J. | en_US |
dc.contributor.author | Kim, J. H. | en_US |
dc.contributor.author | Mauro, D. M. | en_US |
dc.date.accessioned | 2006-09-11T19:44:22Z | |
dc.date.available | 2006-09-11T19:44:22Z | |
dc.date.issued | 1992-02 | en_US |
dc.identifier.citation | Thomas, J. J.; Kim, J. H.; Mauro, D. M.; (1992). "4-(4-nitrobenzyl)pyridine tests for alkylating agents following chemical oxidative activation." Archives of Environmental Contamination and Toxicology 22(2): 219-227. <http://hdl.handle.net/2027.42/48076> | en_US |
dc.identifier.issn | 0090-4341 | en_US |
dc.identifier.issn | 1432-0703 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/48076 | |
dc.description.abstract | A chemical activation system (CAS) designed to mimic the mammalian mixed-function oxidase enzymes was found to activate target compounds to reactive electrophiles. Activated compounds were assayed by reaction with 4-(4-nitrobenzyl)pyridine (NBP). A model nucleophile of 7-alkylguanine of nucleic acids, NBP produces a violet color following alkylation. Twenty compounds from several chemical classes were tested. The test generally gave positive and negative responses where expected. Two compounds, trichloroethylene and diethylnitrosamine, exhibited a linear Beer's law relationship in the concentration range tested. A high degree of linear correlation (r>0.97) was obtained for these compounds. Other compounds showed varying degrees of linear correlation from high correlation (r=0.94) to weak correlation (r=0.44). The CAS-NBP assay results were compared to bacterial mutagenicity and animal carcinogenicity test results when information was available. A good correlation (r=0.80) existed between direct alkylating activity and direct mutagenicity. Similar correlations existed between NBP alkylation following activation and mutagenicity following microsomal activation (r=0.73). Also, different correlations were observed between carcinogenicity and NBP alkylation following activation (r=0.69) and without activation (r=0.38). | en_US |
dc.format.extent | 751352 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Terrestrial Pollution | en_US |
dc.subject.other | Ecology | en_US |
dc.subject.other | Environment | en_US |
dc.subject.other | Agriculture | en_US |
dc.subject.other | Forestry | en_US |
dc.subject.other | Environment, General | en_US |
dc.subject.other | Soil Science & Conservation | en_US |
dc.subject.other | Waste Management/Waste Technology | en_US |
dc.subject.other | Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution | en_US |
dc.title | 4-(4-nitrobenzyl)pyridine tests for alkylating agents following chemical oxidative activation | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Environmental Chemistry Program, Department of Environmental and Industrial Health, School of Public Health, The University of Michigan, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Florida Institute of Technology, 32901, Melbourne, Florida, USA | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Florida Institute of Technology, 32901, Melbourne, Florida, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/48076/1/244_2004_Article_BF00213289.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00213289 | en_US |
dc.identifier.source | Archives of Environmental Contamination and Toxicology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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