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dc.contributor.authorAmonette, R.en_US
dc.contributor.authorGallo, Marcy E.en_US
dc.contributor.authorLauber, Christianen_US
dc.contributor.authorZak, Donald R.en_US
dc.contributor.authorSinsabaugh, Robert L.en_US
dc.date.accessioned2006-09-11T19:47:20Z
dc.date.available2006-09-11T19:47:20Z
dc.date.issued2004-10en_US
dc.identifier.citationGallo, M.; Amonette, R.; Lauber, C.; Sinsabaugh, R. L.; Zak, D. R.; (2004). "Microbial Community Structure and Oxidative Enzyme Activity in Nitrogen-amended North Temperate Forest Soils." Microbial Ecology 48(2): 218-229. <http://hdl.handle.net/2027.42/48115>en_US
dc.identifier.issn0095-3628en_US
dc.identifier.issn1432-184Xen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/48115
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15546042&dopt=citationen_US
dc.description.abstractLarge regions of temperate forest are subject to elevated atmospheric nitrogen (N) deposition which can affect soil organic matter dynamics by altering mass loss rates, soil respiration, and dissolved organic matter production. At present there is no general model that links these responses to changes in the organization and operation of microbial decomposer communities. Toward that end, we studied the response of litter and soil microbial communities to high levels of N amendment (30 and 80 kg ha −1 yr −1 ) in three types of northern temperate forest: sugar maple/basswood (SMBW), sugar maple/red oak (SMRO), and white oak/black oak (WOBO). We measured the activity of extracellular enzymes (EEA) involved directly in the oxidation of lignin and humus (phenol oxidase, peroxidase), and indirectly, through the production of hydrogen peroxide (glucose oxidase, glyoxal oxidase). Community composition was analyzed by extracting and quantifying phospholipid fatty acids (PLFA) from soils. Litter EEA responses at SMBW sites diverged from those at oak-bearing sites (SMRO, BOWO), but the changes were not statistically significant. For soil, EEA responses were consistent across forests types: phenol oxidase and peroxidase activities declined as a function of N dose (33–73% and 5–41%, respectively, depending on forest type); glucose oxidase and glyoxal oxidase activities increased (200–400% and 150–300%, respectively, depending on forest type). Principal component analysis (PCA) ordinated forest types and treatment responses along two axes; factor 1 (44% of variance) was associated with phenol oxidase and peroxidase activities, factor 2 (31%) with glucose oxidase. Microbial biomass did not respond to N treatment, but nine of the 23 PLFA that formed >1 mol% of total biomass showed statistically significant treatment responses. PCA ordinated forest types and treatment responses along three axes (36%, 26%, 12% of variance). EEA factors 1 and 2 correlated negatively with PLFA factor 1 ( r = −0.20 and −0.35, respectively, n = 108) and positively with PLFA factor 3 ( r = +0.36 and +0.20, respectively, n = 108). In general, EEA responses were more strongly tied to changes in bacterial PLFA than to changes in fungal PLFA. Collectively, our data suggests that N inhibition of oxidative activity involves more than the repression of ligninase expression by white-rot basidiomycetes.en_US
dc.format.extent292684 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Spreinger-Verlagen_US
dc.subject.otherNature Conservationen_US
dc.subject.otherGeoecology/Natural Processesen_US
dc.subject.otherMicrobiologyen_US
dc.subject.otherEcologyen_US
dc.subject.otherLife Sciencesen_US
dc.titleMicrobial Community Structure and Oxidative Enzyme Activity in Nitrogen-amended North Temperate Forest Soilsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumSchool of Natural Resources, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherBiology Department, University of New Mexico, Albuquerque, NM, 87131, USAen_US
dc.contributor.affiliationotherBiology Department, University of New Mexico, Albuquerque, NM, 87131, USAen_US
dc.contributor.affiliationotherEnvironmental Science Department, University of Toledo, Toledo, OH, 43606, USAen_US
dc.contributor.affiliationotherBiology Department, University of New Mexico, Albuquerque, NM, 87131, USAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid15546042en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/48115/1/248_2003_Article_9001.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00248-003-9001-xen_US
dc.identifier.sourceMicrobial Ecologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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