Both TNF receptors are required for direct TNF-mediated cytotoxicity in microvascular endothelial cells
dc.contributor.author | Lucas, Rudolf | en_US |
dc.contributor.author | Garcia, Irene | en_US |
dc.contributor.author | Donati, Yves R. A. | en_US |
dc.contributor.author | Hribar, Marusa | en_US |
dc.contributor.author | Mandriota, Stefano J. | en_US |
dc.contributor.author | Giroud, Christine | en_US |
dc.contributor.author | Buurman, Wim A. | en_US |
dc.contributor.author | Fransen, Lucie | en_US |
dc.contributor.author | Suter, Peter M. | en_US |
dc.contributor.author | Nuñez, Gabriel | en_US |
dc.contributor.author | Pepper, Michael S. | en_US |
dc.contributor.author | Grau, Georges E. | en_US |
dc.date.accessioned | 2006-09-20T15:03:29Z | |
dc.date.available | 2006-09-20T15:03:29Z | |
dc.date.issued | 1998-11 | en_US |
dc.identifier.citation | Lucas, Rudolf; Garcia, Irene; Donati, Yves R. A.; Hribar, Marusa; Mandriota, Stefano J.; Giroud, Christine; Buurman, Wim A.; Fransen, Lucie; Suter, Peter M.; NuÑez, Gabriel; Pepper, Michael S.; Grau, Georges E. (1998)."Both TNF receptors are required for direct TNF-mediated cytotoxicity in microvascular endothelial cells." European Journal of Immunology 28(11): 3577-3586. <http://hdl.handle.net/2027.42/48707> | en_US |
dc.identifier.issn | 0014-2980 | en_US |
dc.identifier.issn | 1521-4141 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/48707 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9842900&dopt=citation | en_US |
dc.description.abstract | The conditions under which tumor necrosis factor-α (TNF) induces apoptosis in primary microvascular endothelial cells (MVEC) were investigated. In the absence of sensitizing agents, TNF induced apoptosis after 3 days of incubation in confluent MVEC. In contrast, upon addition of the transcriptional inhibitor actinomycin D (Act. D), confluence was no longer required and apoptosis occurred already after 16 h. To assess the role of either TNF receptor (TNFR) type in apoptosis, MVEC isolated from mice genetically deficient in TNFR1 ( Tnfr1 ° mice) or TNFR2 ( Tnfr2 ° mice) were incubated with TNF in the presence or absence of Act. D. Under sensitized conditions, Tnfr2 ° MVEC were lysed like controls, whereas Tnfr1 ° MVEC were completely resistant, indicating an exclusive role for TNFR1. In contrast, in the absence of Act. D, confluent monolayers of wild-type cells were lysed by TNF, but both Tnfr1 ° and Tnfr2 ° MVEC were resistant to TNF-mediated toxicity, indicating a requirement for both TNFR types. Overexpression of the anti-apoptotic protein bcl-xL in MVEC led to a protection against the direct, but not the sensitized cytotoxicity of TNF. In conclusion, in pathophysiologically relevant conditions, both TNFR appear to be required for TNF-induced apoptosis in MVEC. | en_US |
dc.format.extent | 154676 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | WILEY-VCH Verlag GmbH | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Microbiology and Immunology | en_US |
dc.title | Both TNF receptors are required for direct TNF-mediated cytotoxicity in microvascular endothelial cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Ann Arbor, USA | en_US |
dc.contributor.affiliationother | Laboratory of Immunopathology, Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, University Medical Center, University of Geneva, Geneva, Switzerland ; Laboratory of Intensive Care, Department of Internal Medicine, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Department of Pathology, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Laboratory of Immunopathology, Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Laboratory of Intensive Care, Department of Internal Medicine, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Department of Morphology, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Laboratory of Immunopathology, Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Department of Surgery, University of Limburg, Maastricht, The Netherlands | en_US |
dc.contributor.affiliationother | Innogenetics, Ghent, Belgium | en_US |
dc.contributor.affiliationother | Laboratory of Immunopathology, Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Department of Morphology, University Medical Center, University of Geneva, Geneva, Switzerland | en_US |
dc.contributor.affiliationother | Laboratory of Immunopathology, Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, University Medical Center, University of Geneva, Geneva, Switzerland ; CNRS – UPRES A6020, Faculty of Medicine, UniversitÉ de la MÉditerranÉe, Marseille, France | en_US |
dc.identifier.pmid | 9842900 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/48707/1/3577_ftp.pdf | en_US |
dc.identifier.doi | 10.1002/(ISSN)1521-4141 | |
dc.identifier.source | European Journal of Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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