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dc.contributor.authorMycek, Mary-Annen_US
dc.date.accessioned2006-12-19T18:59:07Z
dc.date.available2006-12-19T18:59:07Z
dc.date.issued2003-07-21en_US
dc.identifier.citationMycek, Mary-Ann (2003). "Imaging fluorescence lifetime modulation of a ruthenium-based dye in living cells: the potential for oxygen sensing." Journal of Physics D: Applied Physics. 36(14): 1689-1695. <http://hdl.handle.net/2027.42/48916>en_US
dc.identifier.issn0022-3727en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/48916
dc.description.abstractFluorescence lifetime measurements of long excited-state lifetime, oxygen-quenched ruthenium dyes are emerging as methods for intracellular oxygen sensing. Fluorescence lifetime imaging microscopy (FLIM) studies in cells have been reported previously. Many current FLIM systems use high repetition rate (∼107 Hz) lasers optimized for nanosecond lifetime measurements, making measurement of long, microsecond lifetime fluorophores difficult. Here, we present an experimental approach for obtaining a large temporal dynamic range in a FLIM system by using a low repetition rate (101 Hz), high output, nitrogen pumped dye laser and a wide-field, intensified CCD camera for image detection. We explore the feasibility of the approach by imaging the oxygen-sensitive dye tris(2,2′-bipyridyl)dichloro-ruthenium(II) hexahydrate (RTDP) in solution and in living cells. We demonstrate the ability of the system to resolve 60% variations in RTDP fluorescence lifetime upon oxygen cycling in solution. Furthermore, the FLIM system was able to resolve an increase in RTDP fluorescence lifetime in cultured human epithelial cells under diminished oxygen conditions. The technique may be useful in developing methods for quantifying intracellular oxygen concentrations.en_US
dc.format.extent3118 bytes
dc.format.extent285286 bytes
dc.format.mimetypetext/plain
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherIOP Publishing Ltden_US
dc.titleImaging fluorescence lifetime modulation of a ruthenium-based dye in living cells: the potential for oxygen sensingen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelPhysicsen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/48916/2/d31406.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1088/0022-3727/36/14/306en_US
dc.identifier.sourceJournal of Physics D: Applied Physics.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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