Distant regulatory elements in a Sox10-ΒGEO BAC transgene are required for expression of Sox10 in the enteric nervous system and other neural crest-derived tissues
dc.contributor.author | Deal, Karen K. | en_US |
dc.contributor.author | Cantrell, V. Ashley | en_US |
dc.contributor.author | Chandler, Ronald L. | en_US |
dc.contributor.author | Saunders, Thomas L. | en_US |
dc.contributor.author | Mortlock, Douglas P. | en_US |
dc.contributor.author | Southard-Smith, E. Michelle | en_US |
dc.date.accessioned | 2007-01-17T15:54:40Z | |
dc.date.available | 2007-01-17T15:54:40Z | |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Deal, Karen K.; Cantrell, V. Ashley; Chandler, Ronald L.; Saunders, Thomas L.; Mortlock, Douglas P.; Southard-Smith, E. Michelle (2006)."Distant regulatory elements in a Sox10-ΒGEO BAC transgene are required for expression of Sox10 in the enteric nervous system and other neural crest-derived tissues." Developmental Dynamics 9999(9999): NA-NA. <http://hdl.handle.net/2027.42/49298> | en_US |
dc.identifier.issn | 1058-8388 | en_US |
dc.identifier.issn | 1097-0177 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/49298 | |
dc.description.abstract | Sox10 is an essential transcription factor required for development of neural crest-derived melanocytes, peripheral glia, and enteric ganglia. Multiple transcriptional targets regulated by Sox10 have been identified; however, little is known regarding regulation of Sox10 . High sequence conservation surrounding 5′ exons 1 through 3 suggests these regions might contain functional regulatory elements. However, we observed that these Sox10 genomic sequences do not confer appropriate cell-specific transcription in vitro when linked to a heterologous reporter. To identify elements required for expression of Sox10 in vivo, we modified bacterial artificial chromosomes (BACs) to generate a Sox10 ΒGeoBAC transgene. Our approach leaves endogenous Sox10 loci unaltered, circumventing haploinsufficiency issues that arise from gene targeting. Sox10 ΒGeoBAC expression closely approximates Sox10 expression in vivo, resulting in expression in anterior dorsal neural tube at embryonic day (E) 8.5 and in cranial ganglia, otic vesicle, and developing dorsal root ganglia at E10.5. Characterization of Sox10 ΒGeoBAC expression confirms the presence of essential regulatory regions and additionally identifies previously unreported expression in thyroid parafollicular cells, thymus, salivary, adrenal, and lacrimal glands. Fortuitous deletions in independent Sox10 ΒGeoBAC lines result in loss of transgene expression in peripheral nervous system lineages and coincide with evolutionarily conserved regions. Our analysis indicates that Sox10 expression requires the presence of distant cis -acting regulatory elements. The Sox10 ΒGeoBAC transgene offers one avenue for specifically testing the role of individual conserved regions in regulation of Sox10 and makes possible analysis of Sox10+ derivatives in the context of normal neural crest development. Developmental Dynamics, 2006. © 2006 Wiley-Liss, Inc. | en_US |
dc.format.extent | 400823 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley-Liss, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Distant regulatory elements in a Sox10-ΒGEO BAC transgene are required for expression of Sox10 in the enteric nervous system and other neural crest-derived tissues | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pediatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee | en_US |
dc.contributor.affiliationother | Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee | en_US |
dc.contributor.affiliationother | Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee | en_US |
dc.contributor.affiliationother | Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee | en_US |
dc.contributor.affiliationother | Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee ; Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, 529 Light Hall, 2215 Garland Avenue, Nashville, TN 37232-0275 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/49298/1/899_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/dvdy.20769 | en_US |
dc.identifier.source | Developmental Dynamics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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