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dc.contributor.authorRichards, Thomas C.en_US
dc.date.accessioned2007-04-06T17:42:59Z
dc.date.available2007-04-06T17:42:59Z
dc.date.issued1973-12en_US
dc.identifier.citationRichards, Thomas C. (1973)."Histochemical changes in developing mouse liver after administration of phenobarbital Part of a dissertation submitted to the Horace H. Rackham School of Graduate Studies of the University of Michigan in partial fulfillment of the requirement for the degree of Doctor of Philosophy. Supported by N.I.H. grant GM 00312, Department of Anatomy, The University of Michigan, Ann Arbor, Michigan 48104. ." American Journal of Anatomy 138(4): 449-463. <http://hdl.handle.net/2027.42/49667>en_US
dc.identifier.issn0002-9106en_US
dc.identifier.issn1553-0795en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49667
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4148401&dopt=citationen_US
dc.description.abstractThe effects of phenobarbital on development of some microsomal enzymes and neutral lipid were studied in mouse liver during a period of rapid cell differentiation. Specific alterations attributable to phenobarbital were identified in developing liver and used as markers for the onset of certain aspects of cell maturation. This investigation was designed to determine (1) the earliest developmental age at which fetal liver can react to phenobarbital and (2) the nature of the alterations in developing and adult liver. Intraperitoneal injections of phenobarbital were given for three days to 14, 17 and 19 day pregnant mice. Histochemical techniques were used to localize neutral lipid and the activities of NADPH diaphorase, glucose-6-phosphatase, and glucose-6-phosphate dehydrogenase. Total cell lipid was measured in livers from 17- and 19-day pregnant mice and livers from their fetuses. The density of reaction product for NADPH diaphorase and glucose-6-phosphate dehydrogenase increased while glucose-6-phosphatase decreased in adult liver. Similar changes were seen in livers from day-19 fetal animals but not in fetuses of younger ages. Neutral lipid droplets increased in size and number after phenobarbital in fetal mice of day 19 of gestation but not in mice of day 17 of gestation. It was concluded that liver from day-19 fetal mice had gained the competence, lacking in earlier ages, to respond to phenobarbital by increased production of some enzymes and neutral lipid.en_US
dc.format.extent1364858 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleHistochemical changes in developing mouse liver after administration of phenobarbital Part of a dissertation submitted to the Horace H. Rackham School of Graduate Studies of the University of Michigan in partial fulfillment of the requirement for the degree of Doctor of Philosophy. Supported by N.I.H. grant GM 00312, Department of Anatomy, The University of Michigan, Ann Arbor, Michigan 48104.en_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMedicine (General)en_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Anatomy, University of Michigan, Ann Arbor, Michigan 48104en_US
dc.identifier.pmid4148401en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49667/1/1001380404_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/aja.1001380404en_US
dc.identifier.sourceAmerican Journal of Anatomyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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