Show simple item record

dc.contributor.authorWomble, Mark D.en_US
dc.contributor.authorCarlson, Bruce M.en_US
dc.date.accessioned2007-04-06T17:45:11Z
dc.date.available2007-04-06T17:45:11Z
dc.date.issued1986-06en_US
dc.identifier.citationWomble, Mark D.; Carlson, B. M. (1986)."The clustering of acetylcholine receptors and formation of neuromuscular junctions in regenerating mammalian muscle grafts." American Journal of Anatomy 176(2): 191-205. <http://hdl.handle.net/2027.42/49689>en_US
dc.identifier.issn0002-9106en_US
dc.identifier.issn1553-0795en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49689
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3739947&dopt=citationen_US
dc.description.abstractThe present investigation was undertaken to study the relationship between acetylcholine receptor (AchR) clustering and endplate formation within regenerating skeletal muscle grafts. Silver staining of nerves was combined with rhodamine-alpha-bungarotoxin labeling of AchR clusters in heterotopic grafts of the rat soleus muscle. Two major graft procedures were used: whole muscle grafts and grafts which lacked the zone of original motor endplates (MEP-less grafts). These categories were subdivided into standard grafts, where subsequent innervation was allowed, and noninnervated grafts, which were experimentally deprived of innervation. Grafting brought about the death and removal of muscle fibers, followed by regeneration of myotubes within surviving basal lamina sheaths. A transient population of small extra-junctinal AchR clusters spontaneously appears shortly after myotube formation in all four muscle graft types. Early myotubes of whole muscle grafts (both innervated and standard grafts, prior to the time of innervation) also develop presumptive secondary synaptic clefts and large, organized aggregations of AchRs at original synaptic sites. At later times, nerves regenerating into standard whole muscle and MEP-less grafts lead to the formation of numerous ectopic endplates. In whole muscle grafts, endplates may also form at original synaptic sites. Functional graft innervation is achieved in whole muscle and MEP-less grafts as early as 20 days postgrafting. The results of this study support the existence of still-unknown factors associated with the original synaptic site which can direct postsynaptic differentiation independent of innervation. They also demonstrate that functional endplates may form in mammalian muscle grafts at both original synaptic sites and ectopic locations, thus indicating that the zone of original synaptic sites is not necessary for the establishment of numerous functional and morphologically well-differentiated endplates.en_US
dc.format.extent1249093 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleThe clustering of acetylcholine receptors and formation of neuromuscular junctions in regenerating mammalian muscle graftsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMedicine (General)en_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Anatomy and Cell Biology, The University of Michigan, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartment of Anatomy & Cell Biology, University of Michigan, Medical Science II, Ann Arbor, MI 48109 ; Department of Anatomy & Cell Biology, University of Michigan, Medical Science II, Ann Arbor, MI 48109en_US
dc.identifier.pmid3739947en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49689/1/1001760208_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/aja.1001760208en_US
dc.identifier.sourceAmerican Journal of Anatomyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record