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Changes in mesenchymal cell-basal lamina relationships preceding palatal shelf reorientation in the mouse

dc.contributor.authorBrinkley, Linda L.en_US
dc.date.accessioned2007-04-06T17:45:17Z
dc.date.available2007-04-06T17:45:17Z
dc.date.issued1986-07en_US
dc.identifier.citationBrinkley, Linda L. (1986)."Changes in mesenchymal cell-basal lamina relationships preceding palatal shelf reorientation in the mouse." American Journal of Anatomy 176(3): 367-378. <http://hdl.handle.net/2027.42/49690>en_US
dc.identifier.issn0002-9106en_US
dc.identifier.issn1553-0795en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49690
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3739957&dopt=citationen_US
dc.description.abstractTwo specific regions of the future nasal and oral epithelial surfaces of the secondary palatal shelves increase in cell density during shelf reorientation. The relationships of mesenchymal cells to the basal lamina underlying these regions were examined and compared to those of cells underlying adjacent regions which did not change in cell density. CD-1 mouse fetuses were obtained on day 13.5 of gestation. Some palatal shelves were excised immediately and fixed for electron microscopy; other heads were partially dissected and incubated for 4 hr prior to fixation. Although shelf movement is detected only after 6 hr incubation, the shorter time period was selected in order to detect events which precede reorientation. Electron micrographs were taken of the epithelial-mesenchymal interface of nasal and oral regions known to increase in epithelial cell density (active segments) and of nasal and oral regions which did not increase (inactive segments). Several measurements were made in a 500-nm-wide zone delimited on photographic prints. Distinct differences in mesenchymal cell configuration were found between nasal and oral regions. Active and inactive segments of each region also differed. A filamentous layer attached to the undersurface of the lamina densa was observed to vary in thickness and character between regions as well. After 4 hr incubation, differences in mesenchymal cell configuration and ultrastructure of the sublaminar zone were apparent between regions. These results suggest that local epithelial-mesenchymal interactions, possibly mediated by the extracellular matrix, precede shelf reorientation. Whether these changes in mesenchymal cell configuration actually reflect mesenchymal cell activities that are necessary for shelf reorientation remains to be elucidated.en_US
dc.format.extent2812976 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleChanges in mesenchymal cell-basal lamina relationships preceding palatal shelf reorientation in the mouseen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMedicine (General)en_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109en_US
dc.identifier.pmid3739957en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49690/1/1001760309_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/aja.1001760309en_US
dc.identifier.sourceAmerican Journal of Anatomyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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