The role of the mesenchyme in mouse neural fold elevation. II. Patterns of hyaluronate synthesis and distribution in embryos developing in vitro

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dc.contributor.author Morris-Wiman, Joyce en_US
dc.contributor.author Brinkley, Linda L. en_US
dc.date.accessioned 2007-04-06T17:45:59Z
dc.date.available 2007-04-06T17:45:59Z
dc.date.issued 1990-06 en_US
dc.identifier.citation Morris-Wiman, Joyce; Brinkley, Linda L. (1990)."The role of the mesenchyme in mouse neural fold elevation. II. Patterns of hyaluronate synthesis and distribution in embryos developing in vitro." American Journal of Anatomy 188(2): 133-147. <http://hdl.handle.net/2027.42/49697> en_US
dc.identifier.issn 0002-9106 en_US
dc.identifier.issn 1553-0795 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/49697
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2375279&dopt=citation en_US
dc.description.abstract Hyaluronate (HA) distribution patterns were examined in the cranial mesenchyme underlying the mesencephalic neural folds of mouse embryos maintained in roller tube culture. Using standard image-processing techniques, the digitized images of Alcian blue-stained or 3 H-glucosamine-labeled sections digested with an enzyme specific for HA, were subtracted from adjacent, undigested sections. The resultant difference picture images (DPI) accurately depicted the distribution of stained or labeled HA within the cranial mesenchyme. 3 H-glucosamine-labeled HA was distributed uniformly throughout the cranial mesenchyme as 12, 18, and 24 hr of culture. By contrast, the mesenchyme was uniformly stained with Alcian blue at 12 hr, but stain intensity decreased in the central regions of the mesenchyme at 18 and 24 hr. HA distribution patterns were also examined in the cranial mesenchyme of embryos cultured in the presence of diazo-oxo-norleucine (DON), a glutamine analogue that inhibits glycosaminoglycan and glycoprotein synthesis. In DON-treated mesenchyme, Alcian blue staining of HA was decreased from that in controls at 12, 18, and 24 hr. However, incorporation of 3 H-glucosamine into HA was increased. The distribution of labeled HA within treated mesenchyme as 12, 18, and 24 hr resembled that in controls at 12 hr. These results indicate that the distribution of HA within the cranial mesenchyme of normal mouse embryos during neural fold elevation and convergence is not determined solely by regional differences in HA synthesis. We propose that HA distribution patterns result from the expansion of the HA-rich extracellular matrix of the central mesenchyme regions. This expansion may play a major role in fold elevation. These results also suggest that DON treatment reversibly inhibits HA synthesis, since treated mesenchymal cells retain the capability of synthesizing HA when provided with a glucosamine substrate. Patterns of 3 H-glucosamine incorporation by DON-treated mesenchyme are similar to those observed in control mesenchyme prior to mesenchymal expansion at 12 hr. en_US
dc.format.extent 2099244 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Cell & Developmental Biology en_US
dc.title The role of the mesenchyme in mouse neural fold elevation. II. Patterns of hyaluronate synthesis and distribution in embryos developing in vitro en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Medicine (General) en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Anatomy & Cell Biology, The University of Michigan Medical School, Ann Arbor, MI 48109 ; Department of Orthodontics, University of Florida College of medicine, Gainesville, FL 32610 en_US
dc.contributor.affiliationum Department of Anatomy & Cell Biology, The University of Michigan Medical School, Ann Arbor, MI 48109 en_US
dc.identifier.pmid 2375279 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/49697/1/1001880204_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/aja.1001880204 en_US
dc.identifier.source American Journal of Anatomy en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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