Expression patterns of engrailed -like proteins in the chick embryo

Show simple item record

dc.contributor.author Gardner, Charles A. en_US
dc.contributor.author Barald, Kate F. en_US
dc.date.accessioned 2007-04-06T17:46:17Z
dc.date.available 2007-04-06T17:46:17Z
dc.date.issued 1992-04 en_US
dc.identifier.citation Gardner, Charles A.; Barald, Kate F. (1992)."Expression patterns of engrailed -like proteins in the chick embryo." American Journal of Anatomy 193(4): 370-388. <http://hdl.handle.net/2027.42/49700> en_US
dc.identifier.issn 0002-9106 en_US
dc.identifier.issn 1553-0795 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/49700
dc.description.abstract The protein products of both of the identified chick engrailed -like ( En ) genes, chick En-1 and chick En-2 , are localized in cells of the developing brain, mandibular arch, spinal cord, dermatome, and ventral limb bud ectoderm, as demonstrated by labeling with the polyclonal antiserum ΑEnhb-1 developed by Davis et al. (Development 111:281–298, 1991). A subpopulation of cephalic neural crest cells is also En -protein-positive. The monoclonal antibody 4D9 recognizes the chick En-2 gene product exclusively (Patel et al.: Cell 58:955–968, 1989; Davis et al., 1991) and colocalizes with chick En-2 mRNA in the developing head region of the chick embryo as shown by in situ hybridization (Gardner et al.: J. Neurosci. Res. 21:426–437, 1988). In the present study we examine the pattern of ΑEnhb-1 and 4D9 localization throughout the chick embryo from the first appearance of antibody (Ab)-positive cells at stage 8 (Hamburger and Hamilton: J. Morphol. 88:49–92, 1951) through stage 28 (1–5.5 days). We compare the localization patterns of the two Abs to each other, as well as to the localization of the monoclonal Ab, HNK-1, which recognizes many neural crest cells, using double-and triple-label fluorescence immunohistochemistry. Most En protein-positive cells in the path of neural crest cell migration are not HNK-1 positive. In detailed examination of ΑEnhb-1 and 4D9 localization, we find previously undetected patterns of En protein localization in the prechordal plate, hindbrain, myotome, ventral body-wall mesoderm, and extraembryonic membranes. Based upon these observations we propose: (1) that En expression in the mesoderm may be induced through interaction with En expressing cells in the neuroectoderm; (2) that En expression in the head mesenchyme is associated with somitomere 4; and (3) that En expression may be involved in epithelial-mesenchymal cell transformations. © 1992 Wiley-Liss, Inc. en_US
dc.format.extent 2509131 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Cell & Developmental Biology en_US
dc.title Expression patterns of engrailed -like proteins in the chick embryo en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Medicine (General) en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 en_US
dc.contributor.affiliationum Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, MI 48109 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/49700/1/1001930410_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/aja.1001930410 en_US
dc.identifier.source American Journal of Anatomy en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
 Show simple item record

This item appears in the following Collection(s)


Search Deep Blue

Advanced Search

Browse by

My Account

Information

Coming Soon


MLibrary logo