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dc.contributor.authorBeaudoin, Allan R.en_US
dc.contributor.authorKahkonen, Dorothyen_US
dc.date.accessioned2007-04-06T17:56:03Z
dc.date.available2007-04-06T17:56:03Z
dc.date.issued1963-11en_US
dc.identifier.citationBeaudoin, Allan R.; Kahkonen, Dorothy (1963)."The effect of trypan blue on the serum proteins of the fetal rat Supported by research grant AM-05603-03 from the National Institute of Metabolic Diseases, Public Health Service, U. S. Department of health, Education, and Welfare. ." The Anatomical Record 147(3): 387-395. <http://hdl.handle.net/2027.42/49798>en_US
dc.identifier.issn0003-276Xen_US
dc.identifier.issn1097-0185en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49798
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14077651&dopt=citationen_US
dc.description.abstractPregnant rats were injected intraperitoneally with a teratogenic dose of trypan blue (14 mg/100 g or 20 mg/100 g body weight) on the eighth day of gestation. Fetal blood was collected from control and experimental fetuses on the twentieth day of pregnancy. Serum was obtained from pooled blood samples of two to three fetuses. Paper electrophoresis was carried out in a Spinco model R system using barbital buffer at pH 8.6. The paper strips were subsequently stained with bromphenol blue and their density measured in a Spinco Analytrol to determine the relative concentration of each component. Total protein was measured in a Bausch and Lomb Serum Protein Meter. The predominant malformations seen in abnormal fetuses were apparent anophthalmia, 59%; hydrocephalus, 37%; and exencephaly, 19%. Other malformations were noted but with less frequency. Twenty-day fetuses from trypan blue treated mothers had a significantly lower total protein concentration (1.50 g/100 ml) than control fetuses (1.95 g/100 ml). A comparison of the absolute protein concentration of the serum fractions revealed significant differences between control and experimental fetuses in beta globulin, alpha-1 globulin, and albumin. In each instance the absolute concentration was less in the fraction from fetuses of trypan blue treated mothers. Fetal gamma, alpha-2, and alpha-3 globulins were not affected by the maternal treatment.en_US
dc.format.extent748809 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleThe effect of trypan blue on the serum proteins of the fetal rat Supported by research grant AM-05603-03 from the National Institute of Metabolic Diseases, Public Health Service, U. S. Department of health, Education, and Welfare.en_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Anatomy, The University of Michigan Medical School, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Anatomy, The University of Michigan Medical School, Ann Arbor, Michigan ; Medical Student Research Fellow.en_US
dc.identifier.pmid14077651en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49798/1/1091470310_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/ar.1091470310en_US
dc.identifier.sourceThe Anatomical Recorden_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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