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dc.contributor.authorBaker, Burton L.en_US
dc.contributor.authorMidgley, A. Reesen_US
dc.contributor.authorGersten, Brian E.en_US
dc.contributor.authorYu, Ya-Yenen_US
dc.date.accessioned2007-04-06T17:57:39Z
dc.date.available2007-04-06T17:57:39Z
dc.date.issued1969-06en_US
dc.identifier.citationBaker, Burton L.; Midgley, A. Rees; Gersten, Brian E.; Yu, Ya-Yen (1969)."Differentiation of growth hormone- and prolactin-containing acidophils with peroxidase-labeled antibody Supported in part by USPHS research grants HD 03159-01A1 and HD 02193, and a grant from the University of Michigan Horace H. Rackham School of Graduate Studies. ." The Anatomical Record 164(2): 163-171. <http://hdl.handle.net/2027.42/49814>en_US
dc.identifier.issn0003-276Xen_US
dc.identifier.issn1097-0185en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49814
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5784107&dopt=citationen_US
dc.description.abstractIn order to verify the concept that growth hormone and prolactin are contained in two different populations of acidophils, sections of Bouin-fixed rat hypophyses were stained immunochemically. For this purpose the histochemical demonstration of peroxidase was utilized after sequential application to the tissue section of rabbit antiserum to human growth hormone (or antiserum to rat prolactin) followed by application of peroxidase-labeled sheep antiserum to rabbit gamma-globulin. It was found that growth hormone cells and prolactin cells, when revealed immunochemically, corresponded structurally to cell types that could be differentiated with reasonable certainty in sections stained with the Masson trichrome procedure. When delineated immunochemically, growth hormone cells were larger and more densely arranged in the adult male than in the intact female; they exhibited little change in the female after ovariectomy. In contrast, prolactin cells were large and frequent in the female hypophysis but were small and less frequent in the male and in the female after ovariectomy. By double-staining, growth hormone and prolactin cells were differentiated in the same section. It was concluded that (a) growth hormone and prolactin are contained in, and presumably secreted by, two different populations of acidophils; and (b) the Masson procedure permits a reasonably accurate differentiation of the two cell types.en_US
dc.format.extent920456 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleDifferentiation of growth hormone- and prolactin-containing acidophils with peroxidase-labeled antibody Supported in part by USPHS research grants HD 03159-01A1 and HD 02193, and a grant from the University of Michigan Horace H. Rackham School of Graduate Studies.en_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Anatomy and Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48104en_US
dc.contributor.affiliationumDepartments of Anatomy and Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48104en_US
dc.contributor.affiliationumDepartments of Anatomy and Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48104en_US
dc.contributor.affiliationumDepartments of Anatomy and Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48104en_US
dc.identifier.pmid5784107en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49814/1/1091640204_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/ar.1091640204en_US
dc.identifier.sourceThe Anatomical Recorden_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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