Immunochemical staining of the rat adenohypophysis in organ culture
Pakurar, Alice S.; Kahn, Raymond H.; Conklin, James L.
Pakurar, Alice S.; Kahn, Raymond H.; Conklin, James L. (1975)."Immunochemical staining of the rat adenohypophysis in organ culture This investigation was supported by National Institutes of Health Training Grant GM00312 from the National Institute of General Medical Sciences and U. S. Public Health Service Grant AM06918. A portion of this work was presented in abstract from at the 81st meeting of the American Association of Anatomists (Anat. Rec., 160 : 436, 1968). This work was submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The University of Michigan. ." The Anatomical Record 181(1): 131-147. <http://hdl.handle.net/2027.42/49829>
AbstractRat anterior pituitaries were cytologically studied following cultivation in organ culture, with and without the addition of hypothalamic and cortical extracts. Although five distinct cell types could be identified with classical stains in the uncultivated glands, the peroxidase-labeled antibody technique (using antibodies against STH, LTH, FSH, LH and TSH) showed that not all of the immune-specific cell types were being identified with the classical stains. This discrepancy was magnified following culture as chromophilic cells seen with classic stains decreased in number with an increase in culture time. The peroxidase technique, however, revealed that all cells remained constant in type and number regardless of time in culture. While the addition of either hypothalamic or cortical extract to the culture medium produced cytological alterations demonstrated by the classical dyes, the antibody technique showed no such alterations. Such a comparison of staining techniques emphasizes the hazards of relying solely on histological procedures to reveal the hormonal activity of the pituitary gland.
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