Show simple item record

dc.contributor.authorJaques, Sandforden_US
dc.contributor.authorTobes, Michael C.en_US
dc.date.accessioned2007-04-06T18:03:44Z
dc.date.available2007-04-06T18:03:44Z
dc.date.issued1986-08en_US
dc.identifier.citationJaques, Sandford; Tobes, Michael C. (1986)."Morphologic and biochemical variability of tissue and cultured cells from human pheochromocytoma." Journal of Cellular Physiology 128(2): 261-270. <http://hdl.handle.net/2027.42/49875>en_US
dc.identifier.issn0021-9541en_US
dc.identifier.issn1097-4652en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49875
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3733889&dopt=citationen_US
dc.description.abstractPrimary cell cultures from 18 human pheochromocytomas were maintained in culture for 10 to 12 days and characterized. The cell yields ranged from 1.0 to 60.1 × 10 6 cells/g wet weight of tissue. Cell size, as determined by histofluorescent microscopy, varied as much as seven-fold among cells derived from a given tumor and ten-fold between cells from all tumors. Cell catecholamine content, norepinephrine (NE) plus epinephrine, ranged from 0.4 to 89.5 nmol/10 6 cells at day 5 in culture and did not correlate with catecholamine content of the tissue from which the cells were obtained. Cell catecholamine content decreased with time in culture, but this decrease could not be related to a change in cell viability, the type of media used, an inability to convert dopamine to NE, or an alteration in the uptake of 3 H-NE. Cellular uptake of 1.0 ΜM 3 H-NE varied as much as 230-fold between all cell dispersions. The basal and acetylcholine stimulated release of both preloaded 3 H-NE and the endogenous catecholamines was quite variable. There was no correlation between the release rate, either basal or stimulated, of preloaded 3 H-NE and the endogenous catecholamines. This study represents the largest existing data base on culturing cells from these tumors and describes many of the morphologic and biochemical characteristics of this cell system.en_US
dc.format.extent1667507 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleMorphologic and biochemical variability of tissue and cultured cells from human pheochromocytomaen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelKinesiology and Sportsen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Nuclear Medicine, Department of Internal Medicine, The University of Michigan Medical Center, Ann Arbor, Michigan 48109 ; Division of Nuclear Medicine, Department of Internal Medicine, The University of Michigan Medical Center, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumDivision of Nuclear Medicine, Department of Internal Medicine, The University of Michigan Medical Center, Ann Arbor, Michigan 48109en_US
dc.identifier.pmid3733889en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49875/1/1041280218_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jcp.1041280218en_US
dc.identifier.sourceJournal of Cellular Physiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record