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dc.contributor.authorStoolman, Lloyd M.en_US
dc.contributor.authorWang, Tai-Lingen_US
dc.contributor.authorSitu, Ruien_US
dc.contributor.authorVarani, Jamesen_US
dc.date.accessioned2007-04-06T18:04:32Z
dc.date.available2007-04-06T18:04:32Z
dc.date.issued1993-03en_US
dc.identifier.citationStoolman, L. M.; Wang, Tai-Ling; Situ, Rui; Varani, J. (1993)."Regulation of fibronectin and laminin binding activity in cultured human lymphoblastic cell lines." Journal of Cellular Physiology 154(3): 593-600. <http://hdl.handle.net/2027.42/49883>en_US
dc.identifier.issn0021-9541en_US
dc.identifier.issn1097-4652en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49883
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8436606&dopt=citationen_US
dc.description.abstractThe current study shows that a clonal derivative of the Jurkat cell line up-regulates both the avidity and density of the Α 6/Β1 receptor in response to phorbol 12-myristate 13-acetate (PMA). This derivative attaches to fibronectin and, to a lesser degree, laminin constitutively. Adhesion and spreading are dramatically up-regulated following treatment with PMA. The response on fibronectin peaks within 4 hours, is insensitive to cyclohexaminde, can be blocked by monoclonal antibodies (Mabs) to the Β1 and Α 5 subunits of the Β1 family of integrins, and is not associated with increased expression of the Α 5 or Β1 epitopes at the cell surface. In contrast, the response on laminin is biphasic. The early phase parallels the response on fibronectin. The second phase peaks after 48–72 hours of treatment with PMA, is sensitive to cycloheximide, can be blocked by Mabs to the Β1 and Α 6 subunits, and is associated with increased expression of the Α 6 epitope. Both the density independent and dependent responses to PMA in Jurkat cells are blocked by the protein kinase inhibitor staurosporine. The HSB-2, CEM, Molt-4, and HPB-ALL T-lymphoblastic cell lines also up-regulate attachment to fibronectin and laminin following treatment with PMA. All four lines constitutively attach to fibronectin and show rapid up-regulation of attachment following treatment with PMA. None of the lines attach to laminin prior to PMA treatment; however, specific adhesion developed after 4–120 hours of treatment. The most mature lines (Jurkat and HPB-ALL) up-regulated adhesion on laminin more rapidly than the less phenotypically mature lines (CEM, Molt-4, and HSB-2). In summary, clonal derivatives of the Jurkat cell line up-regulated attachment to laminin through protein kinase dependent increases in Α /Β1 receptor avidity and density. In addition, the expression of functional receptors for laminin is linked to developmental maturity in a series of T-lymphoblastic cell lines. © 1993 Wiley-Liss, Inc.en_US
dc.format.extent934316 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleRegulation of fibronectin and laminin binding activity in cultured human lymphoblastic cell linesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelKinesiology and Sportsen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48 109-0602 ; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48 109-0602en_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48 109-0602en_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48 109-0602en_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48 109-0602en_US
dc.identifier.pmid8436606en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49883/1/1041540318_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jcp.1041540318en_US
dc.identifier.sourceJournal of Cellular Physiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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