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dc.contributor.authorCaldwell, Jerryen_US
dc.contributor.authorEmerson, Stephen G.en_US
dc.date.accessioned2007-04-06T18:05:01Z
dc.date.available2007-04-06T18:05:01Z
dc.date.issued1994-05en_US
dc.identifier.citationCaldwell, Jerry; Emerson, Stephen G. (1994)."IL-1Α and TNFΑ act synergistically to stimulate production of myeloid colony-stimulating factors by cultured human bone marrow stromal cells and cloned stromal cell strains." Journal of Cellular Physiology 159(2): 221-228. <http://hdl.handle.net/2027.42/49888>en_US
dc.identifier.issn0021-9541en_US
dc.identifier.issn1097-4652en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49888
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7512974&dopt=citationen_US
dc.description.abstractHuman bone marrow stromal cells repond to stimulation by the monokines IL-1 and TNF by producing colony-stimulating factors such as GM-CSF and G-CSF. In this study we show that IL-1Α and TNFΑ act synergistically to stimulate GM-CSF and G-CSF production by cultured marrow stromal cells. We further show that IL-1Α and TNFΑ synergistically stimulate production of GM-CSF and G-CSF by a clonal stroma-derived cell strain. Although IL-1 and TNF share many of the same biological activities, we show that IL-1Α and TNFΑ have an unequal ability to induce myeloid-CSF production by both cultures, with IL-1Α being the more potent inducer. We found that induction by IL-1Α and TNFΑ was independent of cell proliferation. The effect of IL-1Α and TNFΑ on production of the two myeloid-CSFs by the clonal cells was significantly greater than the unfractionated passaged stromal cultures, having the greater effect on G-CSF production. The clonally derived stromal cells constitutively produced colony-stimulating activity, in particular GM-CSF, at levels easily detected by ELISA. These findings show that, in addition to the overlapping and additive activities of IL-1Α and TNFΑ, they can interact synergistically. Our findings further suggest that a small subpopulation of stroma cells may be the major producer of G-CSF in the marrow microenvironment during immune response. © 1994 wiley-Liss, Inc.en_US
dc.format.extent878078 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleIL-1Α and TNFΑ act synergistically to stimulate production of myeloid colony-stimulating factors by cultured human bone marrow stromal cells and cloned stromal cell strainsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelKinesiology and Sportsen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumGraduate Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumDepartment of Internal Medicine and Pediatrics, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Internal Medicine and Pediatrics, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.identifier.pmid7512974en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49888/1/1041590205_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jcp.1041590205en_US
dc.identifier.sourceJournal of Cellular Physiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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