Understanding the dynamics of cellular responsiveness to modifications of metabolic substrates in perifusion
dc.contributor.author | Brand, Rhonda M. | en_US |
dc.contributor.author | Lyons, Richard H. | en_US |
dc.contributor.author | Midgley, A. Rees | en_US |
dc.date.accessioned | 2007-04-06T18:05:07Z | |
dc.date.available | 2007-04-06T18:05:07Z | |
dc.date.issued | 1994-07 | en_US |
dc.identifier.citation | Brand, Rhonda M.; Lyons, R. H.; Midgley, A. Rees (1994)."Understanding the dynamics of cellular responsiveness to modifications of metabolic substrates in perifusion." Journal of Cellular Physiology 160(1): 10-16. <http://hdl.handle.net/2027.42/49889> | en_US |
dc.identifier.issn | 0021-9541 | en_US |
dc.identifier.issn | 1097-4652 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/49889 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8021289&dopt=citation | en_US |
dc.description.abstract | A novel microperifusion system with capabilities for continuous, real-time, potentiometric monitoring of extracellular hydrogen ion concentration has been used to define the response of HeLa cells to abrupt changes in extracellular energy sources or introduction of an inhibitor of glycolysis. Glycolytic inhibition, induced by removal of glucose or introduction of iodoacetate, each led to a rapid, continuous decrease in acid release. The response to iodoacetate took longer than removal of glucose, perhaps due to the time required for binding and activation. Once inhibition began, however, the rate of change was greater than following glucose removal. Conversely, recovery time following iodoacetate inhibition was much slower than with glucose removal. Unlike the response to short-term glucose depletion, a second pulse of iodoacetate resulted in a faster response followed by an even longer recovery time. The response to switching between glucose and glutamine began almost without evident delay. The response patterns revealed that HeLa cells prefer glutamine to glucose, but, in the presence of both energy sources, some glucose continues to be used. In summary, these results indicate that continuous, real-time monitoring of the kinetics of hydrogen-ion release can be used to gain new insights into the dynamics of cellular response to perturbations of extracellular energy sources. © 1994 Wiley-Liss, Inc. | en_US |
dc.format.extent | 827865 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Understanding the dynamics of cellular responsiveness to modifications of metabolic substrates in perifusion | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Kinesiology and Sports | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Reproductive Sciences Program, the University of Michigan, Ann Arbor, Michigan 48109-0404 ; Bioengineering Program, the University of Michigan, Ann Arbor, Michigan 48109-0404 | en_US |
dc.contributor.affiliationum | Reproductive Sciences Program, the University of Michigan, Ann Arbor, Michigan 48109-0404 ; Department of Biochemistry, the University of Michigan, Ann Arbor, Michigan 48109-0404 | en_US |
dc.contributor.affiliationum | Reproductive Sciences Program, the University of Michigan, Ann Arbor, Michigan 48109-0404 ; Bioengineering Program, the University of Michigan, Ann Arbor, Michigan 48109-0404 ; National Center for Infertility Research at Michigan, the University of Michigan, Ann Arbor, Michigan 48109-0404 ; Reproductive Sciences Program, the University of Michigan, Ann Arbor, Michigan 48109-0404 | en_US |
dc.identifier.pmid | 8021289 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/49889/1/1041600103_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jcp.1041600103 | en_US |
dc.identifier.source | Journal of Cellular Physiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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