Genetic factors influencing murine hematopoietic productivity in culture
dc.contributor.author | Phillips, Robert L. | en_US |
dc.contributor.author | Couzens, Matthew S. | en_US |
dc.contributor.author | Van Zant, Gary | en_US |
dc.date.accessioned | 2007-04-06T18:05:19Z | |
dc.date.available | 2007-04-06T18:05:19Z | |
dc.date.issued | 1995-07 | en_US |
dc.identifier.citation | Phillips, Robert L.; Couzens, Matthew S.; Van Zant, Gary (1995)."Genetic factors influencing murine hematopoietic productivity in culture." Journal of Cellular Physiology 164(1): 99-107. <http://hdl.handle.net/2027.42/49891> | en_US |
dc.identifier.issn | 0021-9541 | en_US |
dc.identifier.issn | 1097-4652 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/49891 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7790403&dopt=citation | en_US |
dc.description.abstract | In order to study a previously described genetic difference manifested in stem cell kinetics of specific mouse strains, effects of this putative gene, stk , were measured on growth and expansion of stem and progenitor cell populations ex vivo. Bone marrow cells from each of two inbred mouse strains, C57BL/6J and DBA/2J, were placed into separate bioreactor cultures perfused continuously with growth medium containing erythropoietin (Epo), interleukin-3 (IL-3), granulocyte-macrphage colony stimulating factor (GM-CSF), and Kit ligand as well as 5% CO 2 . Expansion of cell numbers reached 20-fold for DBA/2J and 10-fold for C57BL/6J marrow within about 1 week of culture. Significant production was also seen of colonyforming unit (CFU)-GM (up nine-fold from input levels) just prior to the cell production peak, and, importantly, moderate expansion of day 12 colony-forming unit-spleen (CFU-S; two- to threefold) occurred as well, although CFU-S production peaked at a relatively short 4 days. CFU-S and CFU-GM levels declined rapidly in culture, either because of unfavorable growth conditions or terminal differentiation. Attempts to remove toxic metabolites by increasing the media perfusion rate resulted in a boost in cell expansion capability by DBA/2J marrow. In bioreactors in which stromal cells were established before marrow inoculation, there was greater expansion of CFU-S (especially by DBA/2J) and CFU-GM, although total cell yield appeared to be unaffected, perhaps because the maximum cell density had already been reached. The relative high potential for CFU-S expansion measured in DBA/2J marrow over that of C57BL/6J will be useful in following genetic contributions to bone marrow production capacity. © 1995 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1060796 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Genetic factors influencing murine hematopoietic productivity in culture | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Kinesiology and Sports | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Aastrom Biosciences, Inc., Ann Arbor, Michigan 48106 ; Department of Internal Medicine, Markey Cancer Center, University of Kentucky Medical Center, 800 Rose Street, Lexington, KY 40536-0093 | en_US |
dc.contributor.affiliationother | Aastrom Biosciences, Inc., Ann Arbor, Michigan 48106 | en_US |
dc.identifier.pmid | 7790403 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/49891/1/1041640113_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jcp.1041640113 | en_US |
dc.identifier.source | Journal of Cellular Physiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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