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dc.contributor.authorBernhardt, Robert R.en_US
dc.contributor.authorEaster, Stephen S.en_US
dc.contributor.authorRaymond, Pamela A.en_US
dc.date.accessioned2007-04-06T18:21:13Z
dc.date.available2007-04-06T18:21:13Z
dc.date.issued1988-11-15en_US
dc.identifier.citationBernhardt, Robert; Easter, Stephen S.; Raymond, Pamela A. (1988)."Axons added to the regenerated visual pathway of goldfish establish a normal fiber topography along the age-axis." The Journal of Comparative Neurology 277(3): 420-429. <http://hdl.handle.net/2027.42/50042>en_US
dc.identifier.issn0021-9967en_US
dc.identifier.issn1096-9861en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50042
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3198799&dopt=citationen_US
dc.description.abstractThroughout a goldfish's life, new generations of ganglion cells are added on the retinal margin and their axons extend centrally to occupy predictable positions in the retinotectal pathway, adjacent to their predecessors and subjacent to the pia. The stacking of successive generations of axons defines the age-axis of the pathway. This study examined whether an ordered array of predecessor axons is a prerequisite for the patterned growth of new axons. One optic nerve was crushed intraorbitally and the fish was injected with 3H-thymidine to label the proliferating cells on the retinal margin. The ring of 3H-thymidine-labeled cells separated retina that was present at the time of nerve crush (inside the ring) from new retina added afterward (outside). After a period of 14–16 months postcrush, both tectal lobes received two punctate applications of horseradish peroxidase (HRP), one in the central and the other in peripheral tectum, to retrogradely label contralateral retinal ganglion cell bodies and their axons. The pattern of HRP labeling from the control tectum confirmed earlier work: axons on the central tectum had somata in the central retina, and axons on the peripheral tectum had somata in the peripheral retina. The labeled cells and axons were both in predictable patterns. The somata that were backfilled from applications to the center of the experimental tectum lay inside the radioactive ring and had therefore regenerated their axons. The patterns of their labeled axons in the optic pathway and of their somata in the retina were typical of the regenerated condition as described in earlier studies. The somata backfilled from the periphery of the experimental tectum were outside the radioactive ring and had been added after the optic nerve crush. The patterns of their labeled axons and somata were comparable to the normal pattern. These observations indicate that new axons do not depend on an ordered array of predecessors to reestablish normal order along the age-axis of the pathway.en_US
dc.format.extent1452884 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherAlan R. Liss, Inc.en_US
dc.publisherWiley Periodiocals, Inc.en_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleAxons added to the regenerated visual pathway of goldfish establish a normal fiber topography along the age-axisen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biology, The University of Michigan, Ann Arbor, Michigan 48109–1048en_US
dc.contributor.affiliationumDepartment of Biology, The University of Michigan, Ann Arbor, Michigan 48109–1048en_US
dc.contributor.affiliationumDepartment of Anatomy and Cell Biology, Medical School, The University of Michigan, Ann Arbor, Michigan 48109–1048en_US
dc.identifier.pmid3198799en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50042/1/902770307_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/cne.902770307en_US
dc.identifier.sourceThe Journal of Comparative Neurologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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