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dc.contributor.authorBernhardt, Robert R.en_US
dc.contributor.authorPatel, Chetan K.en_US
dc.contributor.authorWilson, Stephen W.en_US
dc.contributor.authorKuwada, John Y.en_US
dc.date.accessioned2007-04-06T18:22:29Z
dc.date.available2007-04-06T18:22:29Z
dc.date.issued1992-12-08en_US
dc.identifier.citationBernhardt, Robert R.; Patel, Chetan K.; Wilson, Stephen W.; Kuwada, John Y. (1992)."Axonal trajectories and distribution of GABAergic spinal neurons in wildtype and mutant zebrafish lacking floor plate cells." The Journal of Comparative Neurology 326(2): 263-272. <http://hdl.handle.net/2027.42/50054>en_US
dc.identifier.issn0021-9967en_US
dc.identifier.issn1096-9861en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50054
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1479075&dopt=citationen_US
dc.description.abstractThe role of the midline floor plate cells in the neuronal differentiation of the spinal cord was examined by comparing putative GABAergic neurons in wildtype zebrafish embryos with those in cyc-1 mutant embryos. The mutation produces a pleiotropic recessive lethal phenotype and is severe in rostral brain regions, but its direct effect in the caudal hindbrain and the spinal cord is apparently restricted to the depletion of the midline floor plate cells. In wildtype embryos, an antibody against the neurotransmitter GABA labeled the cell bodies, axons, and growth cones of three classes of previously identified neurons; dorsal longitudinal neurons (DoLA), commissural secondary ascending neurons (CoSA), and ventral longitudinal neurons (VeLD). A novel ventral cell type, Kolmer-Agduhr (KA) neurons, was also labeled. In the cyc-1 mutant, abnormalities were observed in some, but not all, of the GABAreactive CoSA, VeLD, and KA axons, while the axonal trajectories of DoLA neurons were not affected. Furthermore, the number of KA cells was reduced in the mutant while the numbers of the other GABAreactive cells were unperturbed. These observations corroborate our earlier hypothesis that the floor plate cells are one of several guidance cues that direct axonal outgrowth near the ventral midline of the spinal cord. They also suggest that the floor plate cells may play a role in the cellular differentiation of the spinal cord of zebrafish embryos. © 1992 Wiley-Liss, Inc.en_US
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dc.format.mimetypeapplication/pdf
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dc.publisherWiley-Liss, Inc.en_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleAxonal trajectories and distribution of GABAergic spinal neurons in wildtype and mutant zebrafish lacking floor plate cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048 ; Neurobiology, ETH-HÖnggerberg, HPM, CH-8093 ZÜrich, Switzerland ; Neurobiology, ETH-HÖnggerberg, HPM, CH-8093 ZÜrich, Switzerlanden_US
dc.contributor.affiliationumDepartment of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048en_US
dc.contributor.affiliationumDepartment of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048en_US
dc.contributor.affiliationotherDevelopment Biology Research Center, Division of Biomedical Sciences, Kings College, London, WC2B United Kingdomen_US
dc.identifier.pmid1479075en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50054/1/903260208_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/cne.903260208en_US
dc.identifier.sourceThe Journal of Comparative Neurologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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