Axonal trajectories and distribution of GABAergic spinal neurons in wildtype and mutant zebrafish lacking floor plate cells
dc.contributor.author | Bernhardt, Robert R. | en_US |
dc.contributor.author | Patel, Chetan K. | en_US |
dc.contributor.author | Wilson, Stephen W. | en_US |
dc.contributor.author | Kuwada, John Y. | en_US |
dc.date.accessioned | 2007-04-06T18:22:29Z | |
dc.date.available | 2007-04-06T18:22:29Z | |
dc.date.issued | 1992-12-08 | en_US |
dc.identifier.citation | Bernhardt, Robert R.; Patel, Chetan K.; Wilson, Stephen W.; Kuwada, John Y. (1992)."Axonal trajectories and distribution of GABAergic spinal neurons in wildtype and mutant zebrafish lacking floor plate cells." The Journal of Comparative Neurology 326(2): 263-272. <http://hdl.handle.net/2027.42/50054> | en_US |
dc.identifier.issn | 0021-9967 | en_US |
dc.identifier.issn | 1096-9861 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50054 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1479075&dopt=citation | en_US |
dc.description.abstract | The role of the midline floor plate cells in the neuronal differentiation of the spinal cord was examined by comparing putative GABAergic neurons in wildtype zebrafish embryos with those in cyc-1 mutant embryos. The mutation produces a pleiotropic recessive lethal phenotype and is severe in rostral brain regions, but its direct effect in the caudal hindbrain and the spinal cord is apparently restricted to the depletion of the midline floor plate cells. In wildtype embryos, an antibody against the neurotransmitter GABA labeled the cell bodies, axons, and growth cones of three classes of previously identified neurons; dorsal longitudinal neurons (DoLA), commissural secondary ascending neurons (CoSA), and ventral longitudinal neurons (VeLD). A novel ventral cell type, Kolmer-Agduhr (KA) neurons, was also labeled. In the cyc-1 mutant, abnormalities were observed in some, but not all, of the GABAreactive CoSA, VeLD, and KA axons, while the axonal trajectories of DoLA neurons were not affected. Furthermore, the number of KA cells was reduced in the mutant while the numbers of the other GABAreactive cells were unperturbed. These observations corroborate our earlier hypothesis that the floor plate cells are one of several guidance cues that direct axonal outgrowth near the ventral midline of the spinal cord. They also suggest that the floor plate cells may play a role in the cellular differentiation of the spinal cord of zebrafish embryos. © 1992 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1161113 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley-Liss, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Axonal trajectories and distribution of GABAergic spinal neurons in wildtype and mutant zebrafish lacking floor plate cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048 ; Neurobiology, ETH-HÖnggerberg, HPM, CH-8093 ZÜrich, Switzerland ; Neurobiology, ETH-HÖnggerberg, HPM, CH-8093 ZÜrich, Switzerland | en_US |
dc.contributor.affiliationum | Department of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048 | en_US |
dc.contributor.affiliationum | Department of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048 | en_US |
dc.contributor.affiliationother | Development Biology Research Center, Division of Biomedical Sciences, Kings College, London, WC2B United Kingdom | en_US |
dc.identifier.pmid | 1479075 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50054/1/903260208_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/cne.903260208 | en_US |
dc.identifier.source | The Journal of Comparative Neurology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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