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Studies on the localization of newly added membrane in growing neurites

dc.contributor.authorFeldman, Eva L.en_US
dc.contributor.authorAxelrod, Danielen_US
dc.contributor.authorSchwartz, Michalen_US
dc.contributor.authorHeacock, Anne M.en_US
dc.contributor.authorAgranoff, Bernard W.en_US
dc.date.accessioned2007-04-06T18:24:50Z
dc.date.available2007-04-06T18:24:50Z
dc.date.issued1981-11en_US
dc.identifier.citationFeldman, E. L.; Axelrod, D.; Schwartz, M.; Heacock, A. M.; Agranoff, B. W. (1981)."Studies on the localization of newly added membrane in growing neurites." Journal of Neurobiology 12(6): 591-598. <http://hdl.handle.net/2027.42/50076>en_US
dc.identifier.issn0022-3034en_US
dc.identifier.issn1097-4695en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50076
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7310397&dopt=citationen_US
dc.description.abstractExplant culture of the adult goldfish retina results in vigorous neuritic growth, provided that the optic nerve has been previously crushed in vivo. The site of new membrane addition in these growing neurites was studied with a membrane-binding lectin Concanavalin A (Con A) in conjunction with a fluorescent indirect antibody method. Explant cultures were labeled with Con A, followed by rabbit antibodies to Con A. This treatment was shown to immobilize Con A receptors while permitting growth. Twenty-four hours later, the washed preparation was treated with fluorescent goat antirabbit antibodies. The bound fluorescent antibodies appeared as a pattern of contiguous fluorescent speckles confined to the area of old growth while regions near the distal end of the neurite were not fluorescent. These results are compatible with the hypothesis that new membrane is added in the region of the growing tip of the neurite.en_US
dc.format.extent1230513 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleStudies on the localization of newly added membrane in growing neuritesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelPsychologyen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumNeuroscience Laboratory and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumNeuroscience Laboratory and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumNeuroscience Laboratory and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumNeuroscience Laboratory and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumNeuroscience Laboratory and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.identifier.pmid7310397en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50076/1/480120607_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/neu.480120607en_US
dc.identifier.sourceJournal of Neurobiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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