Physiological properties of newly formed synapses between sympathetic preganglionic neurons and sympathetic ganglion neurons

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dc.contributor.author Hume, Richard I. en_US
dc.contributor.author Honig, Marcia G. en_US
dc.date.accessioned 2007-04-06T18:25:09Z
dc.date.available 2007-04-06T18:25:09Z
dc.date.issued 1991-04 en_US
dc.identifier.citation Hume, Richard I.; Honig, Marcia G. (1991)."Physiological properties of newly formed synapses between sympathetic preganglionic neurons and sympathetic ganglion neurons." Journal of Neurobiology 22(3): 249-262. <http://hdl.handle.net/2027.42/50079> en_US
dc.identifier.issn 0022-3034 en_US
dc.identifier.issn 1097-4695 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50079
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1653822&dopt=citation en_US
dc.description.abstract We have examined the physiological properties of transmission at newly formed synapses between sympathetic preganglionic neurons and sympathetic ganglion neurons in vitro . Chick neurons were labeled with fluorescent carbocyanine dyes before they were placed into culture (Honig and Hume, 1986), and were studied by making intracellular recordings during the first 2 weeks of coculture. Evoked monosynaptic excitatory postsynaptic potentials (EPSPs) were not observed until 48 h of coculture. Beyond this time, the frequency with which connected pairs could be found did not vary greatly with time. With repetitive stimulation, the evoked monosynaptic EPSPs fluctuated in amplitude from trial to trial and showed depression at frequencies as low as 1 Hz. To gain further information about the quantitative properties of transmission at newly formed synapses, we analyzed the pattern of fluctuations of delayed release EPSPs. In mature systems, delayed release EPSPs are known to represent responses to single quanta, or to the synchronous release of a small number of quanta. For more than half of the connections we studied, the histograms of delayed release EPSPs were extremely broad. This result suggested that either quantal reponses are drawn from a continuous distribution that has a large coefficient of variation or that there are several distinct size classes of quantal responses. The pattern of fluctuation of monosynaptic EPSPs was consistent with both of these possibilities, and was inconsistent with the possibility that monosynaptic EPSPs are composed of quantal subunits with very little intrinsic variation. Although variation in the size of responses to single quanta might arise in a number of ways, one attractive explanation for our results is that the density and type of acetylcholine receptors varies among the different synaptic sites on the surface of developing sympathetic ganglion neurons. en_US
dc.format.extent 1435873 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
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dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Neuroscience, Neurology and Psychiatry en_US
dc.title Physiological properties of newly formed synapses between sympathetic preganglionic neurons and sympathetic ganglion neurons en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbsecondlevel Neurosciences en_US
dc.subject.hlbsecondlevel Psychology en_US
dc.subject.hlbsecondlevel Public Health en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.subject.hlbtoplevel Social Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Biology, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Biology, Natural Science Building, University of Michigan, Ann Arbor, Michigan 48109 en_US
dc.contributor.affiliationum Department of Biology, University of Michigan, Ann Arbor, Michigan 48109 en_US
dc.identifier.pmid 1653822 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50079/1/480220305_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/neu.480220305 en_US
dc.identifier.source Journal of Neurobiology en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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