Local regeneration in the retina of the goldfish
dc.contributor.author | Hitchcock, Peter F. | en_US |
dc.contributor.author | Myhr, Karen J. Lindsey | en_US |
dc.contributor.author | Easter, Stephen S. | en_US |
dc.contributor.author | Mangione-Smith, Rita | en_US |
dc.contributor.author | Jones, Daphne Dwyer | en_US |
dc.date.accessioned | 2007-04-06T18:25:15Z | |
dc.date.available | 2007-04-06T18:25:15Z | |
dc.date.issued | 1992-03 | en_US |
dc.identifier.citation | Hitchcock, Peter F.; Myhr, Karen J. Lindsey; Easter, Stephen S.; Mangione-Smith, Rita; Jones, Daphne Dwyer (1992)."Local regeneration in the retina of the goldfish." Journal of Neurobiology 23(2): 187-203. <http://hdl.handle.net/2027.42/50080> | en_US |
dc.identifier.issn | 0022-3034 | en_US |
dc.identifier.issn | 1097-4695 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50080 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1527527&dopt=citation | en_US |
dc.description.abstract | We have studied regeneration of the retina in the goldfish as a model of regenerative neurogenesis in the central nervous system. Using a transsclearal surgical approach, we excised small patches of retina that were replaced over several weeks by regeneration. Lesioned retinas from three groups of animals were studied to characterize, respectively, the qualitative changes of the retina and surrounding tissues during regeneration, the concomitant cellular proliferation, and the quantitative relationship between regenerated and intact retina. The qualitative and quantitative analyses were done on retinas prepared using standard methods for light microscopy. The planimetric density of regenerated and intact retinal neurons was computed in a group of animals in which the normal planimetric density ranged from high to low. Cell proliferation was investigated by making intraocular injections of 5-bromo-2′-deoxyuridine (BUdr) at various survival times to label proliferating cells and processing retinal sections for BUdr immunocytochemistry. The qualitative analysis showed that the surgery created a gap in the existing retina that was replaced with new retina over the subsequent weeks. The BUdr-labeling experiments demonstrated that the excised retina was replaced by regeneration of new neurons. Neuroepithiallike cells clustered on the wound margin and migrated centripetally, appositionally adding new retina to the old. The quantitative analysis showed that the planimetric density of the regenerated neurons approximated that of the intact ones. | en_US |
dc.format.extent | 1957464 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Local regeneration in the retina of the goldfish | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Ophthalmology and Anatomy and Cell Biology, W.K. Kellogg Eye Center, School of Medicine, the University of Michigan, Ann Arbor, Michigan 48105 ; The Neuroscience Program, the University of Michigan, Ann Arbor, Michigan 48105 ; Department of Ophthalmology and Anatomy and Cell Biology, W.K. Kellogg Eye Center, School of Medicine, the University of Michigan, Ann Arbor, Michigan 48105 | en_US |
dc.contributor.affiliationum | The Neuroscience Program, the University of Michigan, Ann Arbor, Michigan 48105 | en_US |
dc.contributor.affiliationum | The Neuroscience Program, the University of Michigan, Ann Arbor, Michigan 48105 ; Department of Biology, the University of Michigan, Ann Arbor, Michigan 48105 | en_US |
dc.contributor.affiliationum | Department of Biology, the University of Michigan, Ann Arbor, Michigan 48105 | en_US |
dc.contributor.affiliationum | Department of Ophthalmology and Anatomy and Cell Biology, W.K. Kellogg Eye Center, School of Medicine, the University of Michigan, Ann Arbor, Michigan 48105 | en_US |
dc.identifier.pmid | 1527527 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50080/1/480230209_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/neu.480230209 | en_US |
dc.identifier.source | Journal of Neurobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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