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Intracellular partitioning of androgen receptor immunoreactivity in the brain of the male syrian hamster: Effects of castration and steroid replacement

dc.contributor.authorWood, R. I.en_US
dc.contributor.authorNewman, Sarah Winansen_US
dc.date.accessioned2007-04-06T18:25:28Z
dc.date.available2007-04-06T18:25:28Z
dc.date.issued1993-07en_US
dc.identifier.citationWood, R. I.; Newman, S. W. (1993)."Intracellular partitioning of androgen receptor immunoreactivity in the brain of the male syrian hamster: Effects of castration and steroid replacement." Journal of Neurobiology 24(7): 925-938. <http://hdl.handle.net/2027.42/50082>en_US
dc.identifier.issn0022-3034en_US
dc.identifier.issn1097-4695en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50082
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8228970&dopt=citationen_US
dc.description.abstractThe effect of castration and steroid replacement on the intracellular partitioning of the androgen receptor in the brain of the male Syrian hamster was determined using immunocytochemistry. Androgen receptors were visualized using the PG-21 antibody (G. S. Prins) on 40-Μm coronal brain sections from hamsters perfused with 4% paraformaldehyde with or without 0.4% glutaraldehyde. Control studies confirmed antibody specificity in gonad-intact and castrate males. In the normal adult male, androgen receptor immunocytochemistry reveals intense staining confined to the cell nucleus. Castration caused a gradual increase in cytoplasmic labelling within 2 weeks, accompanied by a reduction in nuclear staining intensity in androgen receptor-containing neurons throughout the brain. Cytoplasmic androgen receptor staining was eliminated after treatment of orchidectomized males for only 8 h with exogenous testosterone. Likewise, long-term exposure to testosterone and dihydrotestosterone, a nonaromatizable androgen, maintained nuclear androgen receptor immunoreactivity. However, exposure to low physiologic concentrations of estrogen was not effective in this regard. In addition, we determined that nuclear androgen receptor immunoreactivity decreases in response to inhibitory short-day photoperiod, but without an increase in cytoplasmic immunostaining. This appears to be due to the decrease in androgen production by the testis, rather than a direct photoperiodic effect, because testosterone supplementation to short-day males restored the intensity of nuclear androgen receptor immuno-reactivity to levels comparable to those in the intact male. These findings are compatible with a new model for the intracellular localization of androgen receptors, in which a subset of unoccupied receptors is located in the cell cytoplasm in the absence of ligand. They further demonstrate the repartitioning of such cytoplasmic receptors, thereby confirming and extending previous observations using biochemical techniques on the regulation of neuronal androgen receptors. © 1993 John Wiley & Sons, Inc.en_US
dc.format.extent1546765 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleIntracellular partitioning of androgen receptor immunoreactivity in the brain of the male syrian hamster: Effects of castration and steroid replacementen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelPsychologyen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumReproductive Sciences Program, Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan, 48109-0616 ; Reproductive Sciences Program, Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan, 48109-0616en_US
dc.contributor.affiliationumReproductive Sciences Program, Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan, 48109-0616en_US
dc.identifier.pmid8228970en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50082/1/480240706_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/neu.480240706en_US
dc.identifier.sourceJournal of Neurobiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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