Intracellular partitioning of androgen receptor immunoreactivity in the brain of the male syrian hamster: Effects of castration and steroid replacement

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dc.contributor.author Wood, R. I. en_US
dc.contributor.author Newman, Sarah Winans en_US
dc.date.accessioned 2007-04-06T18:25:28Z
dc.date.available 2007-04-06T18:25:28Z
dc.date.issued 1993-07 en_US
dc.identifier.citation Wood, R. I.; Newman, S. W. (1993)."Intracellular partitioning of androgen receptor immunoreactivity in the brain of the male syrian hamster: Effects of castration and steroid replacement." Journal of Neurobiology 24(7): 925-938. <http://hdl.handle.net/2027.42/50082> en_US
dc.identifier.issn 0022-3034 en_US
dc.identifier.issn 1097-4695 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50082
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8228970&dopt=citation en_US
dc.description.abstract The effect of castration and steroid replacement on the intracellular partitioning of the androgen receptor in the brain of the male Syrian hamster was determined using immunocytochemistry. Androgen receptors were visualized using the PG-21 antibody (G. S. Prins) on 40-Μm coronal brain sections from hamsters perfused with 4% paraformaldehyde with or without 0.4% glutaraldehyde. Control studies confirmed antibody specificity in gonad-intact and castrate males. In the normal adult male, androgen receptor immunocytochemistry reveals intense staining confined to the cell nucleus. Castration caused a gradual increase in cytoplasmic labelling within 2 weeks, accompanied by a reduction in nuclear staining intensity in androgen receptor-containing neurons throughout the brain. Cytoplasmic androgen receptor staining was eliminated after treatment of orchidectomized males for only 8 h with exogenous testosterone. Likewise, long-term exposure to testosterone and dihydrotestosterone, a nonaromatizable androgen, maintained nuclear androgen receptor immunoreactivity. However, exposure to low physiologic concentrations of estrogen was not effective in this regard. In addition, we determined that nuclear androgen receptor immunoreactivity decreases in response to inhibitory short-day photoperiod, but without an increase in cytoplasmic immunostaining. This appears to be due to the decrease in androgen production by the testis, rather than a direct photoperiodic effect, because testosterone supplementation to short-day males restored the intensity of nuclear androgen receptor immuno-reactivity to levels comparable to those in the intact male. These findings are compatible with a new model for the intracellular localization of androgen receptors, in which a subset of unoccupied receptors is located in the cell cytoplasm in the absence of ligand. They further demonstrate the repartitioning of such cytoplasmic receptors, thereby confirming and extending previous observations using biochemical techniques on the regulation of neuronal androgen receptors. © 1993 John Wiley & Sons, Inc. en_US
dc.format.extent 1546765 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Neuroscience, Neurology and Psychiatry en_US
dc.title Intracellular partitioning of androgen receptor immunoreactivity in the brain of the male syrian hamster: Effects of castration and steroid replacement en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbsecondlevel Neurosciences en_US
dc.subject.hlbsecondlevel Psychology en_US
dc.subject.hlbsecondlevel Public Health en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.subject.hlbtoplevel Social Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Reproductive Sciences Program, Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan, 48109-0616 ; Reproductive Sciences Program, Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan, 48109-0616 en_US
dc.contributor.affiliationum Reproductive Sciences Program, Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan, 48109-0616 en_US
dc.identifier.pmid 8228970 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50082/1/480240706_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/neu.480240706 en_US
dc.identifier.source Journal of Neurobiology en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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