Retinal pigmented epithelium does not transdifferentiate in adult goldfish
dc.contributor.author | Knight, Jennifer K. | en_US |
dc.contributor.author | Raymond, Pamela A. | en_US |
dc.date.accessioned | 2007-04-06T18:25:53Z | |
dc.date.available | 2007-04-06T18:25:53Z | |
dc.date.issued | 1995-08 | en_US |
dc.identifier.citation | Knight, Jennifer K.; Raymond, Pamela A. (1995)."Retinal pigmented epithelium does not transdifferentiate in adult goldfish." Journal of Neurobiology 27(4): 447-456. <http://hdl.handle.net/2027.42/50086> | en_US |
dc.identifier.issn | 0022-3034 | en_US |
dc.identifier.issn | 1097-4695 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50086 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7561826&dopt=citation | en_US |
dc.description.abstract | The neural retina of adult goldfish can regenerate from an intrinsic source of proliferative neuronal progenitor cells, but it is not known whether the retina can regenerate by transdifferentiation of the retinal pigmented epithelium (RPE), a phenomenon demonstrated in adult newts. In this study, we asked whether following surgical removal of the neural retina in adult goldfish the RPE was capable of autonomously transdifferentiating and generating new neural retina. The retina was prelabeled by injecting the fluorescent dye Fluoro-Gold (FG) into the eye prior to surgical removal; this procedure ensured that residual retina was labeled with FG and could therefore be distinguished from unlabeled, regenerated retina. To examine the time course of retinal regeneration, and to identify regenerated retinal neurons, the thymidine analogue bromodeoxyuridine was injected intraocularly, and retinas were examined up to 2 months later. We found that the RPE did not transdifferentiate; instead, retinas regenerated only when pieces of residual neural retina were left intact. Under these circumstances, newly regenerated cells derived from proliferating cells intrinsic to the residual neural retina. When retinas were completely removed, as was evident from a lack of FG labeling, there was no retinal regeneration. © 1995 John Wiley & Sons, Inc. | en_US |
dc.format.extent | 974250 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Retinal pigmented epithelium does not transdifferentiate in adult goldfish | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Program and Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Neuroscience Program and Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan 48109 ; Neuroscience Program and Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 7561826 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50086/1/480270402_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/neu.480270402 | en_US |
dc.identifier.source | Journal of Neurobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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