A dual function activity-dependent, muscle-specific enhancer from rat nicotinic acetylcholine receptor δ-subunit gene
dc.contributor.author | Walke, Wade | en_US |
dc.contributor.author | Xiao, Guozhi | en_US |
dc.contributor.author | Goldman, Daniel J. | en_US |
dc.date.accessioned | 2007-04-06T18:26:06Z | |
dc.date.available | 2007-04-06T18:26:06Z | |
dc.date.issued | 1996-11 | en_US |
dc.identifier.citation | Walke, Wade; Xiao, Guozhi; Goldman, Daniel (1996)."A dual function activity-dependent, muscle-specific enhancer from rat nicotinic acetylcholine receptor δ-subunit gene." Journal of Neurobiology 31(3): 359-369. <http://hdl.handle.net/2027.42/50088> | en_US |
dc.identifier.issn | 0022-3034 | en_US |
dc.identifier.issn | 1097-4695 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50088 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8910793&dopt=citation | en_US |
dc.description.abstract | Nicotinic acetylcholine receptors (nAChR) mediate communication between nerve and muscle. The expression of these receptors increases dramatically during muscle development when myoblasts are fusing into multinucleated myotubes. The molecular mechanisms mediating this muscle developmental stage specific expression are not well understood. We report here the identification of nAChR δ-subunit promoter DNA sequences that differentially interact with nuclear proteins isolated from myoblasts, myotubes, and nonmuscle cells. The functional role these sequences play in mediating muscle-specific expression was explored using mutagenesis and enhancer assays. These studies resulted in the identification of a 47-bp muscle-specific enhancer that mediates increased expression of the nAChR δ-submit gene during myotube formation. This enhancer contains an E-box and an element with similarity to the SV40 core enhancer (SVCE). Point mutations throughout this 47-bp enhancer showed that the E-box and the SVCE sequence are both necessary for conferring muscle-specific expression onto a heterologous promoter. Interestingly, this same DNA sequence also functions as an activity-dependent enhancer. © 1996 John Wiley & Sons, Inc. | en_US |
dc.format.extent | 997540 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | A dual function activity-dependent, muscle-specific enhancer from rat nicotinic acetylcholine receptor δ-subunit gene | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 ; Mental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 8910793 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50088/1/8_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-4695(199611)31:3<359::AID-NEU8>3.0.CO;2-9 | en_US |
dc.identifier.source | Journal of Neurobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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