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A dual function activity-dependent, muscle-specific enhancer from rat nicotinic acetylcholine receptor δ-subunit gene

dc.contributor.authorWalke, Wadeen_US
dc.contributor.authorXiao, Guozhien_US
dc.contributor.authorGoldman, Daniel J.en_US
dc.date.accessioned2007-04-06T18:26:06Z
dc.date.available2007-04-06T18:26:06Z
dc.date.issued1996-11en_US
dc.identifier.citationWalke, Wade; Xiao, Guozhi; Goldman, Daniel (1996)."A dual function activity-dependent, muscle-specific enhancer from rat nicotinic acetylcholine receptor δ-subunit gene." Journal of Neurobiology 31(3): 359-369. <http://hdl.handle.net/2027.42/50088>en_US
dc.identifier.issn0022-3034en_US
dc.identifier.issn1097-4695en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50088
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8910793&dopt=citationen_US
dc.description.abstractNicotinic acetylcholine receptors (nAChR) mediate communication between nerve and muscle. The expression of these receptors increases dramatically during muscle development when myoblasts are fusing into multinucleated myotubes. The molecular mechanisms mediating this muscle developmental stage specific expression are not well understood. We report here the identification of nAChR δ-subunit promoter DNA sequences that differentially interact with nuclear proteins isolated from myoblasts, myotubes, and nonmuscle cells. The functional role these sequences play in mediating muscle-specific expression was explored using mutagenesis and enhancer assays. These studies resulted in the identification of a 47-bp muscle-specific enhancer that mediates increased expression of the nAChR δ-submit gene during myotube formation. This enhancer contains an E-box and an element with similarity to the SV40 core enhancer (SVCE). Point mutations throughout this 47-bp enhancer showed that the E-box and the SVCE sequence are both necessary for conferring muscle-specific expression onto a heterologous promoter. Interestingly, this same DNA sequence also functions as an activity-dependent enhancer. © 1996 John Wiley & Sons, Inc.en_US
dc.format.extent997540 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleA dual function activity-dependent, muscle-specific enhancer from rat nicotinic acetylcholine receptor δ-subunit geneen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelPsychologyen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumMental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumMental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumMental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 ; Mental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109en_US
dc.identifier.pmid8910793en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50088/1/8_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/(SICI)1097-4695(199611)31:3<359::AID-NEU8>3.0.CO;2-9en_US
dc.identifier.sourceJournal of Neurobiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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