The metabolism of homogenates of the mouse epididymis

Abstract

This study has attempted an evaluation of the in vitro metabolic characteristics of the epididymis of the mouse and a definition of areas for further research pursuant to the elucidation of the role of this organ in the process of sperm maturation and storage. Optimal conditions were ascertained for the manometric measurement of total respiration and for the estimation of glycolytic activity by assay of lactate accumulation and phosphate esterification in fluoride poisoned homogenates. Homogenates of mouse kidney were utilized in all experiments for comparative purposes. The in vitro data presented indicate the epididymis to be predominantly oriented to a glycolytic metabolism. It is suggested that this metabolic orientation when considered with the results of other investigators is compatible with a hypothesis for the secretion of lactic acid by the epididymal epithelium into the lumen of the epididymal canal for spermatozoan utilization.