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Denervated single myofibers: Neurite interactions and synaptic molecules

dc.contributor.authorJay, Jeanne C.en_US
dc.contributor.authorBarald, Kate F.en_US
dc.date.accessioned2007-04-06T18:31:25Z
dc.date.available2007-04-06T18:31:25Z
dc.date.issued1989-12en_US
dc.identifier.citationJay, Jeanne C.; Barald, Kate F. (1989)."Denervated single myofibers: Neurite interactions and synaptic molecules." Muscle & Nerve 12(12): 981-992. <http://hdl.handle.net/2027.42/50141>en_US
dc.identifier.issn0148-639Xen_US
dc.identifier.issn1097-4598en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50141
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2622472&dopt=citationen_US
dc.description.abstractAs a first step in defining the molecular cues that may be important for reinnervation of long-term denervated muscle, single adult rat muscle fibers that had been denervated from 2 to 24 months in vivo were maintained in culture for 5 days. Embryonic ventral spinal cord explants were added to some of these cultures. Interactions of neurites with individual short-term (up to 5 months) and long-term (17–24 months) denervated muscle fibers were compared with neurite interactions in cultures of young adult muscle fibers (from 3 to 5-month-old rats) or aged muscle fibers (from 17 to 26-month-old rats). We found the following. (1) Three molecules that are found at the neuromscular junction (NMJ)—acetylcholinesterase (AChE), acetylcholine receptors (AChRs), and gelasmin (an acetylcholine receptor clustering factor that is found enriched at NMJ of adult muscle)—were reduced with increasing periods of denervation but not with aging. (2) The number of neurite contacts at junctional regions of muscle fibers that were formed and maintained on cultured muscle fibers depended on denervation time of the muscle in vivo; very few contacts were made or maintained on long-term denervated fibers. (3) Gelasmin, but not AChE or AChRs, was found at points of neurite contact on all muscle fibers examined, raising the possibility that it may serve as a cue for reinnervation and that its loss from longterm denervated muscle may be, at least in part, involved in the failure of neurite contacts to be made or maintained in culture and possibly in vivo.en_US
dc.format.extent1159433 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleDenervated single myofibers: Neurite interactions and synaptic moleculesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Anatomy and Cell Biology, Program in Neuroscience, University of Michigan Medical School, Ann Arbor, MIen_US
dc.contributor.affiliationumDepartment of Anatomy and Cell Biology, Program in Neuroscience, University of Michigan Medical School, Ann Arbor, MI ; Department of Anatomy and Cell Biology, University of Michigan Medical School, Box 0616, Ann Arbor, MI 48109en_US
dc.identifier.pmid2622472en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50141/1/880121206_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/mus.880121206en_US
dc.identifier.sourceMuscle & Nerveen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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