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dc.contributor.authorAmalfitano, Andreaen_US
dc.contributor.authorChamberlain, Jeffrey S.en_US
dc.date.accessioned2007-04-06T18:34:01Z
dc.date.available2007-04-06T18:34:01Z
dc.date.issued1996-12en_US
dc.identifier.citationAmalfitano, Andrea; Chamberlain, Jeffrey S. (1996)."The mdx-amplification-resistant mutation system assay, a simple and rapid polymerase chain reaction-based detection of the mdx allele." Muscle & Nerve 19(12): 1549-1553. <http://hdl.handle.net/2027.42/50166>en_US
dc.identifier.issn0148-639Xen_US
dc.identifier.issn1097-4598en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50166
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8941268&dopt=citationen_US
dc.description.abstractThe mdx mouse is a murine genetic equivalent of the human X-linked lethal disorder, Duchenne muscular dystrophy (DMD). A number of studies utilizing the mdx mouse have demonstrated the feasibility of gene therapy for this disorder. Many such studies require the ability to determine rapidly the mdx genotype of experimental animals. Previous methods described to identify the mdx allele require multiple manipulations which are technically demanding. We now describe a simple and rapid method to detect the mdx and wild-type alleles in crude mouse DNA samples, by the mdx-amplification-resistant mutation system (ARMS) assay. With this system we correctly identified the mdx status of various transgene-containing animals in a rapid and simple fashion. We discuss the utility of this system for many other studies utilizing the mdx mouse as a model system. © 1996 John Wiley & Sons, Inc.en_US
dc.format.extent523523 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleThe mdx-amplification-resistant mutation system assay, a simple and rapid polymerase chain reaction-based detection of the mdx alleleen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Pediatrics, University of Michigan, Ann Arbor, Michigan ; Department of Human Genetics, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDivision of Pediatrics, University of Michigan, Ann Arbor, Michigan ; Department of Human Genetics, University of Michigan, Ann Arbor, Michigan ; University of Michigan, Department of Human Genetics, 1150 W. Medical Center Drive, Medical Sciences Building II, Rm. 3726, Ann Arbor MI 48109-0618en_US
dc.identifier.pmid8941268en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50166/1/4_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/(SICI)1097-4598(199612)19:12<1549::AID-MUS4>3.0.CO;2-Aen_US
dc.identifier.sourceMuscle & Nerveen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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