The mdx-amplification-resistant mutation system assay, a simple and rapid polymerase chain reaction-based detection of the mdx allele

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dc.contributor.author Amalfitano, Andrea en_US
dc.contributor.author Chamberlain, Jeffrey S. en_US
dc.date.accessioned 2007-04-06T18:34:01Z
dc.date.available 2007-04-06T18:34:01Z
dc.date.issued 1996-12 en_US
dc.identifier.citation Amalfitano, Andrea; Chamberlain, Jeffrey S. (1996)."The mdx-amplification-resistant mutation system assay, a simple and rapid polymerase chain reaction-based detection of the mdx allele." Muscle & Nerve 19(12): 1549-1553. <http://hdl.handle.net/2027.42/50166> en_US
dc.identifier.issn 0148-639X en_US
dc.identifier.issn 1097-4598 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50166
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8941268&dopt=citation en_US
dc.description.abstract The mdx mouse is a murine genetic equivalent of the human X-linked lethal disorder, Duchenne muscular dystrophy (DMD). A number of studies utilizing the mdx mouse have demonstrated the feasibility of gene therapy for this disorder. Many such studies require the ability to determine rapidly the mdx genotype of experimental animals. Previous methods described to identify the mdx allele require multiple manipulations which are technically demanding. We now describe a simple and rapid method to detect the mdx and wild-type alleles in crude mouse DNA samples, by the mdx-amplification-resistant mutation system (ARMS) assay. With this system we correctly identified the mdx status of various transgene-containing animals in a rapid and simple fashion. We discuss the utility of this system for many other studies utilizing the mdx mouse as a model system. © 1996 John Wiley & Sons, Inc. en_US
dc.format.extent 523523 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher John Wiley & Sons, Inc. en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Neuroscience, Neurology and Psychiatry en_US
dc.title The mdx-amplification-resistant mutation system assay, a simple and rapid polymerase chain reaction-based detection of the mdx allele en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Neurosciences en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Division of Pediatrics, University of Michigan, Ann Arbor, Michigan ; Department of Human Genetics, University of Michigan, Ann Arbor, Michigan en_US
dc.contributor.affiliationum Division of Pediatrics, University of Michigan, Ann Arbor, Michigan ; Department of Human Genetics, University of Michigan, Ann Arbor, Michigan ; University of Michigan, Department of Human Genetics, 1150 W. Medical Center Drive, Medical Sciences Building II, Rm. 3726, Ann Arbor MI 48109-0618 en_US
dc.identifier.pmid 8941268 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50166/1/4_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/(SICI)1097-4598(199612)19:12<1549::AID-MUS4>3.0.CO;2-A en_US
dc.identifier.source Muscle & Nerve en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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