Rearrangement of the 5S ribosomal RNA gene clusters during the development and replication of the macronucleus in Tetrahymena thermophila

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dc.contributor.author Allen, Sally Lyman en_US
dc.contributor.author Ervin, Paul R. en_US
dc.contributor.author White, Theodore C. en_US
dc.contributor.author McLaren, Ning C. en_US
dc.date.accessioned 2007-04-06T18:34:58Z
dc.date.available 2007-04-06T18:34:58Z
dc.date.issued 1984 en_US
dc.identifier.citation Allen, Sally Lyman; Ervin, Paul R.; White, Theodore C.; McLaren, Ning C. (1984)."Rearrangement of the 5S ribosomal RNA gene clusters during the development and replication of the macronucleus in Tetrahymena thermophila ." Developmental Genetics 5(4): 181-200. <http://hdl.handle.net/2027.42/50175> en_US
dc.identifier.issn 0192-253X en_US
dc.identifier.issn 1520-6408 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50175
dc.description.abstract The organization of the 5S rRNA genes in the MACronuclear genome of Tetrahymena thermophila was examined during MAC development and replication. The 5S genes are arranged in several tandem arrays of alternating transcribed and spacer sequences in both MICronucleus and MAC. The number of EcoRI fragments bearing 5S gene clusters is similar in MIC and MAC. Most fragments occur in both the MIC and newly formed MAC genomes, a few being MIC-limited and a few MAC-limited. The same rearrangements are seen in the MACs of all four caryonides of a mating pair, and most rearrangements are seen in the newly formed MACs of different inbred strains. During replication of the MAC about half the fragments bearing 5S gene clusters disappear in different cell lines, and new fragments containing 5S genes appear. These fragments differ in size from those present in the MIC or newly formed MAC. These alterations occur in the MACs of all strains except strain B, which is more resistant to vegetative rearrangement. The losses and gains of fragments occur during clonal propagation of cell lines. The process begins by 35 fissions following conjugation, but once an alteration occurs, it is stably propagated. Clonal variation occurs with respect to which losses and gains occur, although a nonrandom distribution is seen among cell clones. We conclude that the alterations in MAC fragment size occur at two stages in the life cycle of Tetrahymena. The first stage occurs during conjugation, when the MAC develops from the MIC. The second stage becomes manifest during vegetative growth, when DNA replication occurs in the MAC and daughter molecules are distributed “amitotically” to daughter nuclei. The two-stage character to MAC alterations for the 5S genes is interpreted in terms of the two steps previously described for MAC differentiation: determination and phenotypic assortment. Possible molecular mechanisms are also discussed. en_US
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dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
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dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Genetics en_US
dc.title Rearrangement of the 5S ribosomal RNA gene clusters during the development and replication of the macronucleus in Tetrahymena thermophila en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Biological Chemistry en_US
dc.subject.hlbsecondlevel Genetics en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Division of Biological Sciences, The University of Michigan, Ann Arbor, MI 48109 ; Division of Biological Sciences, The University of Michigan, Ann Arbor, MI 48109 en_US
dc.contributor.affiliationum Division of Biological Sciences, The University of Michigan, Ann Arbor, MI 48109 en_US
dc.contributor.affiliationum Division of Biological Sciences, The University of Michigan, Ann Arbor, MI 48109 en_US
dc.contributor.affiliationum Division of Biological Sciences, The University of Michigan, Ann Arbor, MI 48109 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50175/1/1020050402_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/dvg.1020050402 en_US
dc.identifier.source Developmental Genetics en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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