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dc.contributor.authorJasinskiene, Nijoleen_US
dc.contributor.authorJasinskas, Algimantasen_US
dc.contributor.authorLangmore, John P.en_US
dc.date.accessioned2007-04-06T18:35:24Z
dc.date.available2007-04-06T18:35:24Z
dc.date.issued1995en_US
dc.identifier.citationJasinskiene, Nijole; Jasinskas, Algimantas; Langmore, John P. (1995)."Embryonic regulation of histone ubiquitination the sea urchin." Developmental Genetics 16(3): 278-290. <http://hdl.handle.net/2027.42/50179>en_US
dc.identifier.issn0192-253Xen_US
dc.identifier.issn1520-6408en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50179
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7796537&dopt=citationen_US
dc.description.abstractWe have used quantitative 2-D protein electrophoresis and immunoprecipitation to study the patterns of histone ubiquitination at 10 h and 36 h of embryonic development in Strongylocentrotus purpuratus. Variants csH2A, ΑH2A, ΒH2A, ΓH2A, ΔHA, H2AF./Z, ΑH2B, ΒH2B, and ΓH2B showed up to sevenfold differences in level of monoubiquitination between variants, and individual variants showed up to sixfold changes during development. At 36 h of embryogenesis, the late variants were less ubiquitinated than the early variants, althoug h the overall level of ubiquitination was appreciably greater than at 10 h. Antiubiquitin antibodies were used to precipitate formaldehyde-fixed chromatin fragments in order to estimate the degree of ubiquitination of the early histone genes. The 5′ regulatory region of the active H3 gene appeared to be at least twice as ubiquitinated as the adjacent upstream spacer. However, the absolute level of ubiquitination of the early histone gene repeat seemed to be independent of transcriptional activity. These results show that variant-specific ubiquitination of histones is a part of the developmental program in sea urchin embryos, but is not clearly correlated with transcriptional activity of the early histone genes, except perhaps in the regulatory regions. © 1995 Wiley-Liss, Inc.en_US
dc.format.extent1363751 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherGeneticsen_US
dc.titleEmbryonic regulation of histone ubiquitination the sea urchinen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumBiophysics Research Division and Department of Biology, University of Michigan, Ann Arboren_US
dc.contributor.affiliationumBiophysics Research Division and Department of Biology, University of Michigan, Ann Arboren_US
dc.contributor.affiliationumBiophysics Research Division and Department of Biology, University of Michigan, Ann Arbor ; Biophysics Research Division, University of Michigan, 930 North University, Ann Arbor, MI 48109-1055en_US
dc.identifier.pmid7796537en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50179/1/1020160308_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/dvg.1020160308en_US
dc.identifier.sourceDevelopmental Geneticsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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