Embryonic regulation of histone ubiquitination the sea urchin

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dc.contributor.author Jasinskiene, Nijole en_US
dc.contributor.author Jasinskas, Algimantas en_US
dc.contributor.author Langmore, John P. en_US
dc.date.accessioned 2007-04-06T18:35:24Z
dc.date.available 2007-04-06T18:35:24Z
dc.date.issued 1995 en_US
dc.identifier.citation Jasinskiene, Nijole; Jasinskas, Algimantas; Langmore, John P. (1995)."Embryonic regulation of histone ubiquitination the sea urchin." Developmental Genetics 16(3): 278-290. <http://hdl.handle.net/2027.42/50179> en_US
dc.identifier.issn 0192-253X en_US
dc.identifier.issn 1520-6408 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50179
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7796537&dopt=citation en_US
dc.description.abstract We have used quantitative 2-D protein electrophoresis and immunoprecipitation to study the patterns of histone ubiquitination at 10 h and 36 h of embryonic development in Strongylocentrotus purpuratus. Variants csH2A, ΑH2A, ΒH2A, ΓH2A, ΔHA, H2AF./Z, ΑH2B, ΒH2B, and ΓH2B showed up to sevenfold differences in level of monoubiquitination between variants, and individual variants showed up to sixfold changes during development. At 36 h of embryogenesis, the late variants were less ubiquitinated than the early variants, althoug h the overall level of ubiquitination was appreciably greater than at 10 h. Antiubiquitin antibodies were used to precipitate formaldehyde-fixed chromatin fragments in order to estimate the degree of ubiquitination of the early histone genes. The 5′ regulatory region of the active H3 gene appeared to be at least twice as ubiquitinated as the adjacent upstream spacer. However, the absolute level of ubiquitination of the early histone gene repeat seemed to be independent of transcriptional activity. These results show that variant-specific ubiquitination of histones is a part of the developmental program in sea urchin embryos, but is not clearly correlated with transcriptional activity of the early histone genes, except perhaps in the regulatory regions. © 1995 Wiley-Liss, Inc. en_US
dc.format.extent 1363751 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Genetics en_US
dc.title Embryonic regulation of histone ubiquitination the sea urchin en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Biological Chemistry en_US
dc.subject.hlbsecondlevel Genetics en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Biophysics Research Division and Department of Biology, University of Michigan, Ann Arbor en_US
dc.contributor.affiliationum Biophysics Research Division and Department of Biology, University of Michigan, Ann Arbor en_US
dc.contributor.affiliationum Biophysics Research Division and Department of Biology, University of Michigan, Ann Arbor ; Biophysics Research Division, University of Michigan, 930 North University, Ann Arbor, MI 48109-1055 en_US
dc.identifier.pmid 7796537 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50179/1/1020160308_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/dvg.1020160308 en_US
dc.identifier.source Developmental Genetics en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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