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dc.contributor.authorBarchfeld-Rothschild, C. C.en_US
dc.contributor.authorMedzihradsky, Fedoren_US
dc.date.accessioned2007-04-06T18:40:04Z
dc.date.available2007-04-06T18:40:04Z
dc.date.issued1987en_US
dc.identifier.citationBarchfeld-Rothschild, C. C.; Medzihradsky, F. (1987)."Heterogeneity of opioid receptor binding in brain slices." Journal of Neuroscience Research 18(2): 358-365. <http://hdl.handle.net/2027.42/50218>en_US
dc.identifier.issn0360-4012en_US
dc.identifier.issn1097-4547en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50218
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2891857&dopt=citationen_US
dc.description.abstractA methodological approach was established for the study of ligand binding to multiple opioid receptors in slices from rat brain striatum. Specific binding of radiolabeled opiates was resolved from total binding with enantiomers or excess unlabeled ligand. Equilibrium binding of triated etorphine, dihydromorphine, and ethylketocyclazocine, and competitive displacement of [ 3 H]dihydromorphine by the unlabeled opiates were used to assess both high and low affinity receptor sites. The high-affenity binding components of the radiolabeled opiates were characterized by linear Scatchard plots, K d values of 2.8–3.7 nM, and binding site densities of 180-297 fmol/mg protein. The displacement of [ 3 H]etorphine by morphine and ethylketocyclazocine displayed Hill coefficients of 0.62 and 0.47, respectively, and revealed receptor sites with much lower affinities than those described by the direct binding of these opiates. On the other hand, both morphine and ethylketocyclazocine displaced [ 3 H]dihydromorphine with similar high potencies (apparent K d s, 3-4 nM). The results support the feasibility of using brain slices as a cellular preparation to study opioid receptor mechanisms.en_US
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dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleHeterogeneity of opioid receptor binding in brain slicesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelPsychologyen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Biological Chemistry and Pharmacology, The University of Michigan Medical School, Ann Arboren_US
dc.contributor.affiliationumDepartments of Biological Chemistry and Pharmacology, The University of Michigan Medical School, Ann Arbor ; Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, MI 48109en_US
dc.identifier.pmid2891857en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50218/1/490180214_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jnr.490180214en_US
dc.identifier.sourceJournal of Neuroscience Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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